PRODUCT CODE: ER1803-57

MCU Antibody (ER1803-57)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of MCU on different lysates using anti-MCU antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: A549 <br />
  Lane 3: mouse spleen tissue<br />
  Lane 2: HL-60 <br />
  Lane 4: mouse brain tissue
  • Western blot analysis of MCU on different lysates using anti-MCU antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: A549 <br />
  Lane 3: mouse spleen tissue<br />
  Lane 2: HL-60 <br />
  Lane 4: mouse brain tissue
  • ICC staining MCU in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MCU in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MCU in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-MCU antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MCU antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MCU antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MCU antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-MCU antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with MCU antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of MCU on different lysates using anti-MCU antibody at 1/500 dilution.
Positive control:
Lane 1: A549
Lane 3: mouse spleen tissue
Lane 2: HL-60
Lane 4: mouse brain tissue

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

MCU Antibody (ER1803-57)

Immunogen

Recombinant protein within human mcu aa 50-250.

Host

Rabbit

Positive Control

A549, HL-60, mouse spleen tissue lysates, mouse brain tissue lysates, A431, HT-29, rat cerebellum tissue, human lung cancer tissue, mouse brain tissue, human colon tissue, human esophagus tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

Predicted band size 40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50 -1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

MCU

SYNONYMS

C109A_HUMAN antibody; C10orf42 antibody; Calcium uniporter protein mitochondrial antibody; Ccdc109a antibody; Coiled-coil domain-containing protein 109A antibody; HsMCU antibody; Mitochondrial Calcium Uniporter antibody

SEQUENCE SIMILARITIES

Belongs to the MCU (TC 1.A.77) family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by CaMK2 in heart leads to increased MCU current. The regulation of MCU by CaMK2 is however subject to discussion: another group was unable to reproduce these results. Phosphorylated on tyrosines by PTK2B/PYK2, promoting oligomerization.

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex. Activity is regulated by MICU1 and MICU2. At low Ca2+ levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca2+ levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells.