PRODUCT CODE: 0806-5

MCAM(CD146) Antibody (0806-5)

Applications

  • WB

REACTIVITY

  • Mouse

  • Rat

Western blot analysis on melanoma using anti-CD146 polyclonal antibody.
  • Western blot analysis on melanoma using anti-CD146 polyclonal antibody.
Western blot analysis on melanoma using anti-CD146 polyclonal antibody.

Applications

  • WB

REACTIVITY

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

MCAM(CD146) Antibody (0806-5)

Immunogen

Peptide

Host

Rabbit

Positive Control

Melanoma

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

113 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

Cell surface glycoprotein MUC18

GENE NAME

MCAM

SYNONYMS

MCAM

TISSUE SPECIFICITY

Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

SUBCELLULAR LOCATION

Membrane; Single-pass type I membrane protein.

FUNCTION

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.