PRODUCT CODE: ER1803-04

macroH2A.1 Antibody (ER1803-04)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: mouse pancreas <br />
 Lane 2: SiHa<br />
 Lane 3: PC-3M<br />
  • Western blot analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: mouse pancreas <br />
 Lane 2: SiHa<br />
 Lane 3: PC-3M<br />
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SiHa cells with macroH2A.1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution.
Positive control:
Lane 1: mouse pancreas
Lane 2: SiHa
Lane 3: PC-3M

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

macroH2A.1 Antibody (ER1803-04)

Immunogen

Recombinant protein within human macroh2a.1 aa 200-400.

Host

Rabbit

Positive Control

Mouse pancreas tissue, SiHa, PC-3M, human colon tissue, human placenta tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Core histone macro-H2A.1

GENE NAME

MACROH2A1

SYNONYMS

Histone macroH2A1, mH2A1, H2A/y, H2AFY, MACROH2A1

TISSUE SPECIFICITY

Widely expressed.

POST-TRANSLATIONAL MODIFICATION

Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin.

SUBCELLULAR LOCATION

Nucleus. Chromosome. Note=Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.

FUNCTION

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin.; [Isoform 1]: Binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation. Increases the expression of genes involved in redox metabolism, including SOD3.; [Isoform 2]: Represses SOD3 gene expression.