Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
Lysozyme Monoclonal Antibody (EM1901-98)
Immunogen
Recombinant protein within human lysozyme aa 1-120.
Host
Mouse
Positive Control
HL-60 cell lysates, human spleen tissue, human stomach carcinoma tissue, A549.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A5A8
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein G affinity purified.
MOLECULAR WEIGHT
14 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB:1:500-1:2,000
-
IHC-P:1:200-1:1,000
-
FC:1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Lysozyme
SYNONYMS
1 4 beta N acetylmuramidase C antibody; 1 antibody; 4-beta-N-acetylmuramidase C antibody; EC 3.2.1.17 antibody; LYSC_HUMAN antibody; Lysosyme antibody; Lysozyme (renal amyloidosis) antibody; Lysozyme C antibody; Lysozyme C precursor antibody; LYZ antibody; LZM antibody; Renal amyloidosis antibody
SEQUENCE SIMILARITIES
Belongs to the glycosyl hydrolase 22 family.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
Lysozyme, also known as muramidase or N-acetylmuramide glycanhydrolase, is an antimicrobial enzyme produced by animals that forms part of the innate immune system. Lysozyme is a glycoside hydrolase that catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan, which is the major component of gram-positive bacterial cell wall. Lysozyme is abundant in secretions including tears, saliva, human milk, and mucus. It is also present in cytoplasmic granules of the macrophages and the polymorphonuclear neutrophils (PMNs). Large amounts of lysozyme can be found in egg white. C-type lysozymes are closely related to alpha-lactalbumin in sequence and structure, making them part of the same glycoside hydrolase family 22. In humans, the C-type lysozyme enzyme is encoded by the LYZ gene. In certain cancers (especially myelomonocytic leukemia) excessive production of lysozyme by cancer cells can lead to toxic levels of lysozyme in the blood. High lysozyme blood levels can lead to kidney failure and low blood potassium, conditions that may improve or resolve with treatment of the primary malignancy. Serum lysozyme is much less specific for diagnosis of sarcoidosis than serum angiotensin converting enzyme; however, since it is more sensitive, it is used as a marker of sarcoidosis disease activity and is suitable for disease monitoring in proven cases.