PRODUCT CODE: EM1901-61

LYRIC Monoclonal Antibody (EM1901-61)

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of LYRIC on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: MCF-7 cell lysate<br />
Lane 2: Jurkat cell lysate<br />
Lane 3: Hela cell lysate
  • Western blot analysis of LYRIC on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: MCF-7 cell lysate<br />
Lane 2: Jurkat cell lysate<br />
Lane 3: Hela cell lysate
  • Flow cytometric analysis of LYRIC was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-61, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of LYRIC on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

LYRIC Monoclonal Antibody (EM1901-61)

Immunogen

Recombinant protein within human lyric aa 1-250.

Host

Mouse

Positive Control

MCF-7 cell lysate, Jurkat cell lysate, Hela cell lysate, K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

6-F4

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G affinity purified.

MOLECULAR WEIGHT

64 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB:1:500-1:2,000

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Protein LYRIC

GENE NAME

MTDH

SYNONYMS

MTDH

TISSUE SPECIFICITY

Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Cell junction, tight junction. Nucleus, nucleolus. Cytoplasm, perinuclear region. Note=In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.

FUNCTION

Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.