PRODUCT CODE: 0902-5

LPA receptor 1 Antibody (0902-5)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Mouse

Western blot analysis on mouse liver using anti-LPA-receptor I polyclonal antibody.
  • Western blot analysis on mouse liver using anti-LPA-receptor I polyclonal antibody.
Western blot analysis on mouse liver using anti-LPA-receptor I polyclonal antibody.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

LPA receptor 1 Antibody (0902-5)

Immunogen

Peptide

Host

Rabbit

Positive Control

Mouse liver

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

41 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

TARGET

UNIPROT #

PROTEIN NAME

Lysophosphatidic acid receptor 1

GENE NAME

Lpar1

SYNONYMS

LPA receptor 1,LPA-1

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family.

TISSUE SPECIFICITY

Detected in brain corpus callosum, medulla oblongata, cerebellum and sciatic nerve. Detected in heart.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.

SUBCELLULAR LOCATION

Cell surface. Cell membrane; Multi-pass membrane protein. Endosome. Note=Prior to LPA treatment found predominantly at the cell surface. Internalized after LPA treatment. Colocalizes with RALA in endocytic vesicles after LPA treatment.

FUNCTION

Receptor for lysophosphatidic acid (LPA). Plays a role in the reorganization of the actin cytoskeleton, cell migration, differentiation and proliferation, and thereby contributes to the responses to tissue damage and infectious agents. Activates downstream signaling cascades via the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Signaling inhibits adenylyl cyclase activity and decreases cellular cAMP levels. Signaling triggers an increase of cytoplasmic Ca(2+) levels. Activates RALA; this leads to the activation of phospholipase C (PLC) and the formation of inositol 1,4,5-trisphosphate. Signaling mediates activation of down-stream MAP kinases. Contributes to the regulation of cell shape. Promotes Rho-dependent reorganization of the actin cytoskeleton in neuronal cells and neurite retraction. Promotes the activation of Rho and the formation of actin stress fibers. Promotes formation of lamellipodia at the leading edge of migrating cells via activation of RAC1. Through its function as lysophosphatidic acid receptor, plays a role in chemotaxis and cell migration, including responses to injury and wounding. Plays a role in triggering inflammation in response to bacterial lipopolysaccharide (LPS) via its interaction with CD14. Promotes cell proliferation in response to lysophosphatidic acid. Required for normal skeleton development. May play a role in osteoblast differentiation. Required for normal brain development. Required for normal proliferation, survival and maturation of newly formed neurons in the adult dentate gyrus. Plays a role in pain perception and in the initiation of neuropathic pain.

CITATIONS

  • Zhao, Guiyun et al.

    Cell surface proteomics analysis indicates a neural lineage bias of rat bone marrow mesenchymal stromal cells. | BioMed Research International [2014]