PRODUCT CODE: ER0403

LMNB2 Antibody (ER0403)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Lamin B2 on different cell lysates using anti-LMNB2 antibody at 1/1000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane2 :Jurkat <br />
 Lane 3: HepG2 <br />
 Lane 4:Raji
  • Western blot analysis of Lamin B2 on different cell lysates using anti-LMNB2 antibody at 1/1000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane2 :Jurkat <br />
 Lane 3: HepG2 <br />
 Lane 4:Raji
  • ICC staining of LMNB2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining of LMNB2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining of LMNB2 in mouse embryonic stem cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-LMNB2 antibody. Counter stained with hematoxylin.
Western blot analysis of Lamin B2 on different cell lysates using anti-LMNB2 antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane2 :Jurkat
Lane 3: HepG2
Lane 4:Raji

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

LMNB2 Antibody (ER0403)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, HepG2, Jurkat, Raji, mouse embryonic stem cell human colon carcinoma tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

68 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

LMNB2

SYNONYMS

LAMB 2 antibody; LAMB2 antibody; Lamin-B2 antibody; LMN 2 antibody; LMN B2 antibody; LMN2 antibody; LMNB 2 antibody; LMNB2 antibody; LMNB2_HUMAN antibody; MGC2721 antibody; RGD1563803 antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

POST-TRANSLATIONAL MODIFICATION

B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

SUBCELLULAR LOCATION

Nucleus inner membrane

FUNCTION

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD), which is a rare childhood disease characterized by loss of subcutaneous fat from the face and trunk.