PRODUCT CODE: M1312-1

Lin28B Mouse Monoclonal Antibody [A2-4-5] (M1312-1)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

Western blot analysis on Lin28B recombinant protein lysates using anti- Lin28B mouse mAb.
  • Western blot analysis on Lin28B recombinant protein lysates using anti- Lin28B mouse mAb.
  • ICC staining Lin28B in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. and counterstained with DAPI in order to highlight the nucleus (blue).
Western blot analysis on Lin28B recombinant protein lysates using anti- Lin28B mouse mAb.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Lin28B Mouse Monoclonal Antibody [A2-4-5] (M1312-1)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

NCCIT, Lin28B recombinant protein

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A2-4-5

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

~27 kDa

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:100-1:500

  • ICC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Lin28B

SYNONYMS

CSDD 2 antibody; CSDD2 antibody; FLJ16517 antibody; Lin 28 homolog B (C. elegans) antibody; Lin 28.2 antibody; Lin-28B antibody; LIN28B antibody; LN28B_HUMAN antibody; Protein lin-28 homolog B antibody

SEQUENCE SIMILARITIES

Belongs to the lin-28 family.

TISSUE SPECIFICITY

Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver. Isoform 1 is only detected in placenta and in moderately and poorly differentiated hepatocellular carcinoma cells (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level). Tends to be up-regulated in triple-negative (ER-,PR-,HER2-) breast tumors, as well as in liver, ovarian, and thyroid carcinomas.

SUBCELLULAR LOCATION

Cytoplasm, nucleus

FUNCTION

LIN28 is thought to regulate the self-renewal of stem cells. In nematodes, the LIN28 homolog lin-28 is a heterochronic gene that determines the onset of early larval stages of developmental events in Caenorhabditis elegans, by regulating the self-renewal of nematode stem cells in the skin (called seam cells) and vulva (called VPCs) during development. In mice, LIN28 is highly expressed in mouse embryonic stem cells and during early embryogenesis.It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Lin28B is high expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC).