PRODUCT CODE: ET1704-26

Lin28 Recombinant Rabbit Monoclonal Antibody [JA10-63] (ET1704-26)

  • Recombinant

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

-
+
Western blot analysis of Lin-28 on NCCIT cells lysates using anti-Lin-28 antibody at 1/500 dilution.
  • Western blot analysis of Lin-28 on NCCIT cells lysates using anti-Lin-28 antibody at 1/500 dilution.
  • Flow cytometric analysis of Hela cells with Lin-28 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Lin-28 on NCCIT cells lysates using anti-Lin-28 antibody at 1/500 dilution.

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Lin28 Recombinant Rabbit Monoclonal Antibody [JA10-63] (ET1704-26)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NCCIT cells lysates, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA10-63

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

23 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IP

  • 1:10-1:50 FC

TARGET

UNIPROT #

PROTEIN NAME

Lin28

SYNONYMS

AL024421 antibody; CSDD1 antibody; CSDD2 antibody; FLJ12457 antibody; Lin 28 antibody; Lin 28 homolog (C. elegans) antibody; Lin 28 homolog A (C. elegans) antibody; Lin 28 homolog A antibody; Lin 28 homolog antibody; Lin-28A antibody; Lin28 antibody; Lin28, C. elegans, homolog of, A antibody; LIN28A antibody; LN28A_HUMAN antibody; Protein lin-28 homolog A antibody; Protein lin-28 homolog B antibody; RNA binding protein lin 28 antibody; Tex17 antibody; ZCCHC1 antibody; Zinc finger CCHC domain containing 1 antibody; Zinc finger CCHC domain containing protein 1 antibody; Zinc finger CCHC domain-containing protein 1 antibody

SEQUENCE SIMILARITIES

Belongs to the lin-28 family.

TISSUE SPECIFICITY

Expressed in embryonic stem cells, placenta and testis. Tends to be up-regulated in HER2-overexpressing breast tumors.

DEVELOPMENTAL STAGE

Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

LIN-28 is a highly conserved, RNA-binding, cytoplasmic protein. It consists of a cold shock domain and retroviral-type (CCHC) zinc finger motifs that were first identified in Caenorhabditis elegans. LIN-28 controls the timing of events during embryonic development and is readily expressed in embryos, embryonic stem cells and embryonal carcinoma cells. The presence of LIN-28 persists in some adult tissues including cardiac and skeletal muscle. In differentiating myoblasts, LIN-28 increases protein sysnthesis efficiency and binds to the growth and differentiation factor IGF-II.