Applications
-
WB
-
IHC-P
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
LAMP2a Antibody (ER1802-99)
Immunogen
Synthetic peptide within c-terminal human lamp2a.
Host
Rabbit
Positive Control
Mouse placenta tissue, human prostate tissue, human kidney tissue, human placenta tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified.
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
LAMP2a
SYNONYMS
CD107 antigen-like family member B antibody; CD107b antibody; LAMP 2 antibody; Lamp 2a antibody; LAMP-2 antibody; LAMP2 antibody; LAMP2_HUMAN antibody; Lysosome-associated membrane glycoprotein 2 antibody; Lysosome-associated membrane protein 2 antibody
SEQUENCE SIMILARITIES
Belongs to the LAMP family.
TISSUE SPECIFICITY
Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is detected in spleen, thymus, prostate, testis, small intestine, colon, skeletal muscle, brain, placenta, lung, kidney, ovary and pancreas and liver. Isoform LAMP-2C is detected in small intestine, colon, heart, brain, skeletal muscle, and at lower levels in kidney and placenta.
POST-TRANSLATIONAL MODIFICATION
O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
SUBCELLULAR LOCATION
Plasma membrane. Endosome. Lysosome.
FUNCTION
Lysosome-associated membrane proteins (LAMP) are glycosylated type I membrane proteins that play a role in the biogenesis of the pigment melanin. LAMP-1 (also designated CD107A) and LAMP-2 (also designated CD107B) are involved in a variety of functions, including cellular adhesion, and are thought to participate in the process of tumor invasion and metastasis. Newly synthesized LAMP-1 and LAMP-2 proteins are sorted at the trans Golgi network and are transported intracellularly via a pathway that is distinct from the clathrin-coated vesicles used for the mannose-6 phosphate receptor. LAMP-1 is expressed on the surface of thrombin-activated but not resting platelets, and it is thought to be involved in the adhesive, prothrombic properties of these cells. Both LAMP-1 and LAMP-2 are involved in maintaining lysosome acidity and protecting the lysosomal membranes from autodigestion, and their expression is increased in patients with lysosomal storage disorders.