PRODUCT CODE: EM50109

Ku70 Monoclonal Antibody (EM50109)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Ku70 on different cell lysates using anti-Ku70 antibody at 1/2000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: HepG2 <br />
 Lane 3: A431 <br />
 Lane 4: D3 <br />
 Lane 5: 293T <br />
 Lane 6: A549 <br />
 Lane 7: COS-1 <br />
 Lane 8: Human kidney <br />
 Lane 9: Human liver <br />
 Lane 10: human brain <br />
 Lane 11: Human thymus <br />
 Lane 12: Human placentae <br />
 Lane 13: Mouse kidney <br />
 Lane 14: Mouse liver <br />
 Lane 15: Mouse thymus <br />
 Lane 16: Mouse testis
  • Western blot analysis of Ku70 on different cell lysates using anti-Ku70 antibody at 1/2000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: HepG2 <br />
 Lane 3: A431 <br />
 Lane 4: D3 <br />
 Lane 5: 293T <br />
 Lane 6: A549 <br />
 Lane 7: COS-1 <br />
 Lane 8: Human kidney <br />
 Lane 9: Human liver <br />
 Lane 10: human brain <br />
 Lane 11: Human thymus <br />
 Lane 12: Human placentae <br />
 Lane 13: Mouse kidney <br />
 Lane 14: Mouse liver <br />
 Lane 15: Mouse thymus <br />
 Lane 16: Mouse testis
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ku70 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Ku70 antibody. Counter stained with hematoxylin.
Western blot analysis of Ku70 on different cell lysates using anti-Ku70 antibody at 1/2000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: A431
Lane 4: D3
Lane 5: 293T
Lane 6: A549
Lane 7: COS-1
Lane 8: Human kidney
Lane 9: Human liver
Lane 10: human brain
Lane 11: Human thymus
Lane 12: Human placentae
Lane 13: Mouse kidney
Lane 14: Mouse liver
Lane 15: Mouse thymus
Lane 16: Mouse testis

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Ku70 Monoclonal Antibody (EM50109)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Hela, HepG2, A431, D3, 293T, A549, COS-1, human kidney tissue, human liver tissue, human brain, human thymus tissue, human placentae tissue, mouse kidney tissue, mouse liver tissue, mouse thymus tissue, mouse testis tissue, human tonsil tissue,human breast cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

D1-A6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:2,000

  • IHC-P

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Ku70

SYNONYMS

5'-deoxyribose-5-phosphate lyase Ku70 antibody; 5'-dRP lyase Ku70 antibody; 70 kDa subunit of Ku antigen antibody; ATP dependent DNA helicase 2 subunit 1 antibody; ATP dependent DNA helicase II 70 kDa subunit antibody; ATP-dependent DNA helicase 2 subunit 1 antibody; ATP-dependent DNA helicase II 70 kDa subunit antibody; CTC box binding factor 75 kDa subunit antibody; CTC box-binding factor 75 kDa subunit antibody; CTC75 antibody; CTCBF antibody; DNA repair protein XRCC6 antibody; G22P1 antibody; Ku 70 antibody; Ku autoantigen p70 subunit antibody; Ku autoantigen, 70kDa antibody; Ku p70 antibody; Ku70 antibody; Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa antibody; Kup70 antibody; Lupus Ku autoantigen protein p70 antibody; ML8 antibody; Thyroid autoantigen 70kD (Ku antigen) antibody; Thyroid autoantigen antibody; Thyroid lupus autoantigen antibody; Thyroid lupus autoantigen p70 antibody; Thyroid-lupus autoantigen antibody; TLAA antibody; X ray repair complementing defective repair in Chinese hamster cells 6 antibody; X-ray repair complementing defective repair in Chinese hamster cells 6 antibody; X-ray repair cross-complementing protein 6 antibody; XRCC 6 antibody; Xrcc6 antibody; XRCC6_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the ku70 family.

DEVELOPMENTAL STAGE

Expression does not increase during promyelocyte differentiation.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.; ADP-ribosylated by PARP3.

SUBCELLULAR LOCATION

Nucleus

FUNCTION

Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V(D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system.In addition to its role in NHEJ, Ku is also required for telomere length maintenance and subtelomeric gene silencing. Ku was originally identified when patients with systemic lupus erythematosus were found to have high levels of autoantibodies to the protein.