PRODUCT CODE: EM50109

Ku70 Monoclonal Antibody (EM50109)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Ku70 on different cell lysates using anti-Ku70 antibody at 1/2000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: HepG2 <br />
Lane 3: A431 <br />
Lane 4: D3 <br />
Lane 5: 293T <br />
Lane 6: A549 <br />
Lane 7: COS-1 <br />
Lane 8: Human kidney <br />
Lane 9: Human liver <br />
Lane 10: human brain <br />
Lane 11: Human thymus <br />
Lane 12: Human placentae <br />
Lane 13: Mouse kidney <br />
Lane 14: Mouse liver <br />
Lane 15: Mouse thymus <br />
Lane 16: Mouse testis
  • Western blot analysis of Ku70 on different cell lysates using anti-Ku70 antibody at 1/2000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: HepG2 <br />
Lane 3: A431 <br />
Lane 4: D3 <br />
Lane 5: 293T <br />
Lane 6: A549 <br />
Lane 7: COS-1 <br />
Lane 8: Human kidney <br />
Lane 9: Human liver <br />
Lane 10: human brain <br />
Lane 11: Human thymus <br />
Lane 12: Human placentae <br />
Lane 13: Mouse kidney <br />
Lane 14: Mouse liver <br />
Lane 15: Mouse thymus <br />
Lane 16: Mouse testis
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ku70 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Ku70 antibody. Counter stained with hematoxylin.
Western blot analysis of Ku70 on different cell lysates using anti-Ku70 antibody at 1/2000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: A431
Lane 4: D3
Lane 5: 293T
Lane 6: A549
Lane 7: COS-1
Lane 8: Human kidney
Lane 9: Human liver
Lane 10: human brain
Lane 11: Human thymus
Lane 12: Human placentae
Lane 13: Mouse kidney
Lane 14: Mouse liver
Lane 15: Mouse thymus
Lane 16: Mouse testis

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Ku70 Monoclonal Antibody (EM50109)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Hela, HepG2, A431, D3, 293T, A549, COS-1, human kidney tissue, human liver tissue, human brain, human thymus tissue, human placentae tissue, mouse kidney tissue, mouse liver tissue, mouse thymus tissue, mouse testis tissue, human tonsil tissue,human breast cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

D1-A6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:2,000

  • IHC-P

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

X-ray repair cross-complementing protein 6

GENE NAME

XRCC6

SYNONYMS

5'-dRP lyase Ku70,CTC75,CTCBF,Ku70,TLAA

SEQUENCE SIMILARITIES

Belongs to the ku70 family.

DEVELOPMENTAL STAGE

Expression does not increase during promyelocyte differentiation.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.; ADP-ribosylated by PARP3.

SUBCELLULAR LOCATION

Nucleus. Chromosome.

FUNCTION

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.