PRODUCT CODE: ER1803-03

Kir3.4 Antibody (ER1803-03)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Kir3.4 on different lysates using anti-Kir3.4 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse heart <br />
 Lane 2: Rat heart<br />
  • Western blot analysis of Kir3.4 on different lysates using anti-Kir3.4 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse heart <br />
 Lane 2: Rat heart<br />
  • ICC staining Kir3.4 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Kir3.4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Kir3.4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with Kir3.4 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Kir3.4 on different lysates using anti-Kir3.4 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse heart
Lane 2: Rat heart

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Kir3.4 Antibody (ER1803-03)

Immunogen

Synthetic peptide within n-terminal human kir3.4.

Host

Rabbit

Positive Control

Mouse heart tissue, rat heart tissue, 293T, HepG2, LOVO, human lung cancer tissue, human pancreas tissue, mouse pancreas tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

G protein-activated inward rectifier potassium channel 4

GENE NAME

KCNJ5

SYNONYMS

GIRK-4, CIR, IRK-4, KCNJ5, GIRK4

SEQUENCE SIMILARITIES

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ5 subfamily.

TISSUE SPECIFICITY

Islets, exocrine pancreas and heart. Expressed in the adrenal cortex, particularly the zona glomerulosa.

SUBCELLULAR LOCATION

Membrane; Multi-pass membrane protein.

FUNCTION

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.