Positive control:
Lane 1: K562 cell lysate
Lane 2: Rat testis tissue lysate
Applications
-
WB
-
IHC
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
KIFAP3 Antibody (ER2001-69)
Immunogen
Recombinant protein within human kifap3 aa 1-200.
Host
Rabbit
Positive Control
K562 cell lysate, rat testis tissue lysate, mouse hippocampus tissue lysates, rat bladder tissue, human liver carcinoma tissue, human thyroid tissue, SHSY5Y.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
Predicted band size: 91 kDa.
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
IHC
-
1:100-1:500
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
KIFAP3
SYNONYMS
FLJ22818 antibody; KAP 3 antibody; KAP-3 antibody; KAP3 antibody; KIF3AP antibody; KIFA3_HUMAN antibody; Kifap3 antibody; Kinesin associated protein 3 antibody; Kinesin-associated protein 3 antibody; SMAP antibody; Smg GDS antibody; Smg GDS associated protein antibody; Smg GDS-associated protein antibody
SUBCELLULAR LOCATION
cytosol, endoplasmic reticulum, Golgi apparatus, Nucleus.
FUNCTION
The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene.