PRODUCT CODE: ER1803-40

KIF3A Antibody (ER1803-40)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of KIF3A on rat testis tissue (1) and Daudi cell (2) lysates using anti-KIF3A antibody at 1/500 dilution.
  • Western blot analysis of KIF3A on rat testis tissue (1) and Daudi cell (2) lysates using anti-KIF3A antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-KIF3A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-KIF3A antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with KIF3A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of KIF3A on rat testis tissue (1) and Daudi cell (2) lysates using anti-KIF3A antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

KIF3A Antibody (ER1803-40)

Immunogen

Recombinant protein within human kif3a aa 100-360.

Host

Rabbit

Positive Control

Rat testis tissue, Daudi, rat cerebellum tissue, mouse testis tissue, A549.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

80 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Kinesin-like protein KIF3A

GENE NAME

KIF3A

SYNONYMS

KIF3A, KIF3

SEQUENCE SIMILARITIES

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin II subfamily.

SUBCELLULAR LOCATION

Cytoplasm, cytoskeleton. Note=Localizes to the subdistal appendage region of the centriole.

FUNCTION

Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitment of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole.