PRODUCT CODE: ER1802-31

Ki67 Antibody (ER1802-31)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ki67 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ki67 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
  • Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ki67 antibody. Counter stained with hematoxylin.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Ki67 Antibody (ER1802-31)

Immunogen

Peptide

Host

Rabbit

Positive Control

Human tonsil tissue, human lung cancer tissue, human colon cancer tissue, human stomach cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

359 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Ki67

SYNONYMS

Antigen identified by monoclonal antibody Ki 67 antibody; Antigen identified by monoclonal antibody Ki-67 antibody; Antigen KI-67 antibody; Antigen KI67 antibody; Antigen Ki67 antibody; KI67_HUMAN antibody; KIA antibody; Marker of proliferation Ki-67 antibody; MIB 1 antibody; MIB antibody; MKI67 antibody; PPP1R105 antibody; Proliferation marker protein Ki-67 antibody; Proliferation related Ki 67 antigen antibody; Protein phosphatase 1 regulatory subunit 105 antibody; RP11-380J17.2 antibody

DEVELOPMENTAL STAGE

Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).