PRODUCT CODE: em1701-97

KHSRP Monoclonal Antibody (EM1701-97)

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of KHSRP on Jurkat cell lysate using anti-KHSRP antibody at 1/500 dilution.
  • Western blot analysis of KHSRP on Jurkat cell lysate using anti-KHSRP antibody at 1/500 dilution.
  • Flow cytometric analysis of HT-29 cells with KHSRP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of KHSRP on Jurkat cell lysate using anti-KHSRP antibody at 1/500 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

KHSRP Monoclonal Antibody (EM1701-97)

Immunogen

Synthetic peptide within human khsrp aa 600-700.

Host

Mouse

Positive Control

Jurkat, HT-29.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A7-B0

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

ProG purified.

MOLECULAR WEIGHT

73 kDa

Isotype

IgM

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Far upstream element-binding protein 2

GENE NAME

KHSRP

SYNONYMS

FUSE-binding protein 2, KSRP, KHSRP, FUBP2

SEQUENCE SIMILARITIES

Belongs to the KHSRP family.

TISSUE SPECIFICITY

Detected in neural and non-neural cell lines.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-193 leads to the unfolding of the unstable KH domain 1, creating a site for 14-3-3 YWHAZ binding, which promotes nuclear localization and impairs the RNA degradation function.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Note=A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites.

FUNCTION

Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.