PRODUCT CODE: ER1706-36

KCNA4 Antibody (ER1706-36)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Kv1.4 on rat brain (1) and mouse brain (2) tissue lysate using anti-Kv1.4 antibody at 1/1,000 dilution.
  • Western blot analysis of Kv1.4 on rat brain (1) and mouse brain (2) tissue lysate using anti-Kv1.4 antibody at 1/1,000 dilution.
  • ICC staining Kv1.4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Kv1.4 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Kv1.4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Kv1.4 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with Kv1.4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Kv1.4 on rat brain (1) and mouse brain (2) tissue lysate using anti-Kv1.4 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

KCNA4 Antibody (ER1706-36)

Immunogen

Peptide

Host

Rabbit

Positive Control

Rat brain tissue, mouse brain tissue, N2A, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

73 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:100

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

KCNA4

SYNONYMS

Voltage gated K+ channel HuKII antibody; Fetal skeletal muscle potassium channel antibody; HBK4 antibody; HK1 antibody; HPCN2 antibody; HUKII antibody; Kcna4 antibody; KCNA4_HUMAN antibody; KCNA4L antibody; KCNA8 antibody; kv1.4 antibody; PCN2 antibody; Potassium channel 2 antibody; Potassium voltage gated channel shaker related subfamily member 4 antibody; Potassium voltage gated channel subfamily A member 4 antibody; Potassium voltage-gated channel subfamily A member 4 antibody; Rapidly inactivating potassium channel antibody; Shaker related potassium channel Kv1.4 antibody; Type A potassium channel antibody; Voltage gated potassium channel HBK4 antibody; Voltage gated potassium channel HK1 antibody; Voltage gated potassium channel subunit Kv1.4 antibody; Voltage-gated K(+) channel HuKII antibody; Voltage-gated potassium channel HBK4 antibody; Voltage-gated potassium channel HK1 antibody; Voltage-gated potassium channel subunit Kv1.4 antibody

SEQUENCE SIMILARITIES

Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.4/KCNA4 sub-subfamily.

TISSUE SPECIFICITY

Expressed in the brain, lens and retina.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.