PRODUCT CODE: ER1803-07

K2P4.1 Antibody (ER1803-07)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of K2P4.1 on different lysates using anti-K2P4.1 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse colon <br />
  Lane 2: Rat kidney
  • Western blot analysis of K2P4.1 on different lysates using anti-K2P4.1 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse colon <br />
  Lane 2: Rat kidney
  • Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-K2P4.1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-K2P4.1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with K2P4.1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of K2P4.1 on different lysates using anti-K2P4.1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse colon
Lane 2: Rat kidney

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

K2P4.1 Antibody (ER1803-07)

Immunogen

Synthetic peptide within human k2p4.1 aa 320-380.

Host

Rabbit

Positive Control

Mouse colon tissue, rat kidney tissue, rat large intestine tissue, HepG2.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

43 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

K2P4.1

SYNONYMS

K2p4.1 antibody; K2P4.1 potassium channel antibody; KCNK 4 antibody; MGC144821 antibody; Potassium channel subfamily K member 4 antibody; Potassium inwardly rectifying channel subfamily K member 4 antibody; TRAAK antibody; TRAAK1 antibody; TRAAKt antibody; TWIK related arachidonic acid stimulated potassium channel protein antibody; Two pore K(+) channel KT4.1 antibody; Two pore K+ channel KT4.1 antibody; Two pore potassium channel KT4.1 antibody

SEQUENCE SIMILARITIES

Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

K2P4.1 (also named TWIK-related arachidonic acid stimulated K+ channel, TRAAK or KCNK4) is a member of the 2-pore (2P) domain K+ channels family that at the moment includes 15 members. These channels show little time or voltage dependence and are considered to be “leaky” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini.