PRODUCT CODE: 0407-9

Junctophilin-2 Antibody (0407-9)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis on mouse heart using anti-JPH-2 polyclonal antibody.
  • Western blot analysis on mouse heart using anti-JPH-2 polyclonal antibody.
Western blot analysis on mouse heart using anti-JPH-2 polyclonal antibody.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Junctophilin-2 Antibody (0407-9)

Immunogen

Peptide

Host

Rabbit

Positive Control

Mouse heart

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

94/74kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2000

TARGET

UNIPROT #

PROTEIN NAME

Junctophilin-2

GENE NAME

JPH2

SYNONYMS

JP-2

SEQUENCE SIMILARITIES

Belongs to the junctophilin family.

TISSUE SPECIFICITY

Specifically expressed in skeletal muscle and heart.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation on Ser-165, probably by PKC, affects RYR1-mediated calcium ion release, interaction with TRPC3, and skeletal muscle myotubule development.; Proteolytically cleaved by calpain in response to cardiac stress. The major cleavage site takes place at the C-terminus and leads to the release of the Junctophilin-2 N-terminal fragment chain (JP2NT).

SUBCELLULAR LOCATION

[Junctophilin-2]: Cell membrane. Note=Accumulates in the nucleus of stressed hearts.

FUNCTION

[Junctophilin-2]: Membrane-binding protein that provides a structural bridge between the plasma membrane and the sarcoplasmic reticulum and is required for normal excitation-contraction coupling in cardiomyocytes. Provides a structural foundation for functional cross-talk between the cell surface and intracellular Ca(2+) release channels by maintaining the 12-15 nm gap between the sarcolemma and the sarcoplasmic reticulum membranes in the cardiac dyads (By similarity). Necessary for proper intracellular Ca(2+) signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release (By similarity). Contributes to the construction of skeletal muscle triad junctions (By similarity).; [Junctophilin-2 N-terminal fragment]: Transcription repressor required to safeguard against the deleterious effects of cardiac stress. Generated following cleavage of the Junctophilin-2 chain by calpain in response to cardiac stress in cardiomyocytes. Following cleavage and release from the membrane, translocates to the nucleus, binds DNA and represses expression of genes implicated in cell growth and differentiation, hypertrophy, inflammation and fibrosis. Modifies the transcription profile and thereby attenuates pathological remodeling in response to cardiac stress. Probably acts by competing with MEF2 transcription factors and TATA-binding proteins.