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PRODUCT CODE: ER1803-65

ITPR2 Antibody (ER1803-65)

Applications

  • Dot-blot

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500.
  • Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500.
  • ICC staining ITPR2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ITPR2 in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ITPR2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with ITPR2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500.

Applications

  • Dot-blot

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ITPR2 Antibody (ER1803-65)

Immunogen

Recombinant protein within human itpr2 aa 1100-1450.

Host

Rabbit

Positive Control

HepG2, HT-29, SiHa, rat kidney tissue, human colon tissue, human breast tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

308 kDa

Isotype

IgG

APPLICATION DILUTION

  • Dot-blot

  • 1:2,000-1:5,000

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50 -1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q14571

PROTEIN NAME

Inositol 1,4,5-trisphosphate receptor type 2

GENE NAME

ITPR2

SYNONYMS

IP3R 2, InsP3R2, Type 2 InsP3 receptor, ITPR2

SEQUENCE SIMILARITIES

Belongs to the InsP3 receptor family.

TISSUE SPECIFICITY

Isoform Short is found in skeletal muscle and heart.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by cAMP-dependent PKA on Ser-937 increases calcium release.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane; Multi-pass membrane protein.

FUNCTION

Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. This release is regulated by cAMP both dependently and independently of PKA (By similarity).