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PRODUCT CODE: ER1803-65

ITPR2 Antibody (ER1803-65)

Applications

  • Dot-blot

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500.
  • Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500.
  • ICC staining ITPR2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ITPR2 in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ITPR2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with ITPR2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500.

Applications

  • Dot-blot

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ITPR2 Antibody (ER1803-65)

Immunogen

Recombinant protein within human itpr2 aa 1100-1450.

Host

Rabbit

Positive Control

HepG2, HT-29, SiHa, rat kidney tissue, human colon tissue, human breast tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

308 kDa

Isotype

IgG

APPLICATION DILUTION

  • Dot-blot

  • 1:2,000-1:5,000

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50 -1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q14571

PROTEIN NAME

ITPR2

SYNONYMS

4 antibody; 5-trisphosphate receptor antibody; Inositol 1 4 5 trisphosphate receptor type 2 antibody; Inositol 1,4,5-trisphosphate receptor type 2 antibody; Inositol 145 trisphosphate receptor type 2 antibody; InsP3 R2 antibody; InsP3R2 antibody; IP3 R2 antibody; IP3 receptor antibody; IP3 receptor isoform 2 antibody; IP3R 2 antibody; IP3R2 antibody; ITPR 2 antibody; Itpr2 antibody; ITPR2_HUMAN antibody; Type 2 inositol 1 4 5 trisphosphate receptor antibody; Type 2 inositol 1 antibody; Type 2 inositol 145 trisphosphate receptor antibody; Type 2 InsP3 receptor antibody

SEQUENCE SIMILARITIES

Belongs to the InsP3 receptor family.

TISSUE SPECIFICITY

Isoform Short is found in skeletal muscle and heart.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by cAMP-dependent PKA on Ser-937 increases calcium release.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane.

FUNCTION

The protein encoded by this gene belongs to the inositol 1,4,5-triphosphate receptor family, whose members are second messenger intracellular calcium release channels. These proteins mediate a rise in cytoplasmic calcium in response to receptor activated production of inositol triphosphate. Inositol triphosphate receptor-mediated signaling is involved in many processes including cell migration, cell division, smooth muscle contraction, and neuronal signaling. This protein is a type 2 receptor that consists of a cytoplasmic amino-terminus that binds inositol triphosphate, six membrane-spanning helices that contribute to the ion pore, and a short cytoplasmic carboxy-terminus. A mutation in this gene has been associated with anhidrosis, suggesting that intracellular calcium release mediated by this protein is required for eccrine sweat production.