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PRODUCT CODE: EM40705

Isocitrate dehydrogenase/IDH1 Monoclonal Antibody (EM40705)

  • Zebrafish

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Zebrafish

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Western blot analysis of IDH1 on different cell lysates using anti-IDH1 antibody at 1/1000 dilution.<br /> Positive control:<br /> Lane 1: Hela<br /> Lane 2: HepG2<br /> Lane 3: A431<br /> Lane 4: MCF-7<br /> Lane 5: A549<br /> Lane 6: Jurkat<br /> Lane 7: Human kidney<br /> Lane 8: Human brain<br /> Lane 9: Human liver
  • Western blot analysis of IDH1 on different cell lysates using anti-IDH1 antibody at 1/1000 dilution.<br /> Positive control:<br /> Lane 1: Hela<br /> Lane 2: HepG2<br /> Lane 3: A431<br /> Lane 4: MCF-7<br /> Lane 5: A549<br /> Lane 6: Jurkat<br /> Lane 7: Human kidney<br /> Lane 8: Human brain<br /> Lane 9: Human liver
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-IDH1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-IDH1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IDH1 antibody. Counter stained with hematoxylin.
Western blot analysis of IDH1 on different cell lysates using anti-IDH1 antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: A431
Lane 4: MCF-7
Lane 5: A549
Lane 6: Jurkat
Lane 7: Human kidney
Lane 8: Human brain
Lane 9: Human liver

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Zebrafish

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Isocitrate dehydrogenase/IDH1 Monoclonal Antibody (EM40705)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

MCF-7, HepG2, Hela, A549, A431, Jurkat, human brain tissue, human liver tissue , human kidney tissue,human colon carcinoma tissue,human breast tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A11-B4

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

47 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:100-1:200

TARGET

UNIPROT #

O75874

PROTEIN NAME

Isocitrate dehydrogenase [NADP] cytoplasmic

GENE NAME

IDH1

SEQUENCE SIMILARITIES

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

POST-TRANSLATIONAL MODIFICATION

Acetylation at Lys-374 dramatically reduces catalytic activity.

SUBCELLULAR LOCATION

Cytoplasm, cytosol. Peroxisome.