PRODUCT CODE: ER1803-17

IP3 Receptor Antibody (ER1803-17)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of IP3 Receptor on Hela cell lysate using anti-IP3 Receptor antibody at 1/500 dilution.
  • Western blot analysis of IP3 Receptor on Hela cell lysate using anti-IP3 Receptor antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IP3 Receptor antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-IP3 Receptor antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with IP3 Receptor antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of IP3 Receptor on Hela cell lysate using anti-IP3 Receptor antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IP3 Receptor Antibody (ER1803-17)

Immunogen

Synthetic peptide within c-terminal human ip3 receptor.

Host

Rabbit

Positive Control

Hela, rat brain tissue, mouse brain tissue, SH-SY-5Y.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

314 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Inositol 1,4,5-trisphosphate receptor type 1

GENE NAME

ITPR1

SYNONYMS

IP3R 1, InsP3R1, Type 1 InsP3 receptor, ITPR1, INSP3R1

SEQUENCE SIMILARITIES

Belongs to the InsP3 receptor family.

TISSUE SPECIFICITY

Widely expressed.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on tyrosine residues.; Ubiquitination at multiple lysines targets ITPR1 for proteasomal degradation. Approximately 40% of the ITPR1-associated ubiquitin is monoubiquitin, and polyubiquitins are both 'Lys-48'- and 'Lys-63'-linked (By similarity).; Phosphorylated by cAMP kinase (PKA). Phosphorylation prevents the ligand-induced opening of the calcium channels. Phosphorylation by PKA increases the interaction with inositol 1,4,5-trisphosphate and decreases the interaction with AHCYL1.; Palmitoylated by ZDHHC6 in immune cells, leading to regulate ITPR1 stability and function.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Endoplasmic reticulum and secretory granules (By similarity).

FUNCTION

Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways (By similarity).