PRODUCT CODE: ER1706-89

iNOS Antibody (ER1706-89)

Applications

  • ICC

  • IHC-P

  • FC

  • WB

REACTIVITY

  • Human

  • Mouse

ICC staining iNOS in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining iNOS in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining iNOS in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-iNOS antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-iNOS antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-iNOS antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of LOVO cells with iNOS antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
ICC staining iNOS in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

  • FC

  • WB

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

iNOS Antibody (ER1706-89)

Immunogen

Peptide

Host

Rabbit

Positive Control

A549, LOVO, human tonsil tissue, human spleen tissue, mouse small intestine tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze/thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

131 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

iNOS

SYNONYMS

HEP-NOS antibody; Hepatocyte NOS antibody; HEPNOS antibody; inducible antibody; Inducible nitric oxide synthase antibody; Inducible NO synthase antibody; Inducible NOS antibody; iNOS antibody; MAC NOS antibody; Macrophage NOS antibody; Nitric oxide synthase 2 inducible antibody; Nitric oxide synthase 2 inducible macrophage antibody; nitric oxide synthase 2A (inducible, hepatocytes) antibody; Nitric oxide synthase antibody; Nitric oxide synthase inducible antibody; nitric oxide synthase, macrophage antibody; NOS 2 antibody; NOS antibody; Nos II antibody; NOS type II antibody; nos2 antibody; NOS2_HUMAN antibody; NOS2A antibody; NOS2A, Inducible, Hepatocyte antibody; Peptidyl-cysteine S-nitrosylase NOS2 antibody

SEQUENCE SIMILARITIES

Belongs to the NOS family.

TISSUE SPECIFICITY

Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.

POST-TRANSLATIONAL MODIFICATION

Polyubiquitinated; mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation.

SUBCELLULAR LOCATION

Cytoskeleton. Nucleus. Cytosol.

FUNCTION

Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.