PRODUCT CODE: ER1706-55

IL4 Antibody (ER1706-55)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of IL4 on recombiant protein lysate using anti-IL4 antibody at 1/1,000 dilution.
  • Western blot analysis of IL4 on recombiant protein lysate using anti-IL4 antibody at 1/1,000 dilution.
  • ICC staining pro IL4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-IL4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IL4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-IL4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL4 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with IL4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of IL4 on recombiant protein lysate using anti-IL4 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IL4 Antibody (ER1706-55)

Immunogen

Recombinant protein.

Host

Rabbit

Positive Control

Recombinant protein lysate, human spleen tissue, human breast cancer tissue, mouse colon tissue, mouse small intestine tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

17 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

IL4

SYNONYMS

B cell growth factor 1 antibody; B cell IgG differentiation factor antibody; B Cell Stimulatory Factor 1 antibody; B-cell stimulatory factor 1 antibody; BCGF 1 antibody; BCGF1 antibody; Binetrakin antibody; BSF-1 antibody; BSF1 antibody; IGG1 induction factor antibody; IL 4 antibody; IL-4 antibody; IL4 antibody; IL4_HUMAN antibody; Il4e12 antibody; Interleukin 4 antibody; Interleukin 4 variant 2 antibody; Interleukin 4, isoform 1 antibody; Interleukin-4 antibody; Lymphocyte stimulatory factor 1 antibody; MGC79402 antibody; Pitrakinra antibody

SEQUENCE SIMILARITIES

Belongs to the IL-4/IL-13 family.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Interleukin-4 (IL-4), also designated B cell stimulatory factor-1, is a glycosylated cytokine secreted by activated T lymphocytes, basophils and mast cells. The secreted IL-4 protein promotes the growth and differentiation of cells that participate in immune defense by favoring such events as the expansion of the Th2 lineage relative to Th1 cells. These T helper cell subsets are defined by their pattern of cytokine secretion: Th1 cells secrete IL-2, TNFβ and IFN-γ, while Th2 cells secrete IL-4, IL-5 and IL-10. Another key immunological function of IL-4 is to induce immunoglobulin class switching. IL-4 has been shown to induce the production of IgE and enhance IgG4 secretion by B cells, but suppress the production of IgM, IgA, IgG1, IgG2, and IgG3. It has been determined that Stat6 is rapidly tyrosine phosphorylated following stimulation of IL-3 or IL-4, but is not detectably phosphorylated following stimulation with IL-2, IL-12 or erythropoietin.

CITATIONS

  • Chen, Ji et al.

    Imbalanced shift of cytokine expression between T helper 1 and T helper 2 (Th1/Th2) in intestinal mucosa of patients with post-infectious irritable bowel syndrome. | Bmc Gastroenterology [2012]