PRODUCT CODE: 0902-2

IL1RL1 Antibody (0902-2)

  • Zebrafish

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Zebrafish

Western blot analysis of IL1RL1 on D3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0902-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of IL1RL1 on D3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0902-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of IL1RL1 on Zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0902-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of IL1RL1 on D3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0902-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IL1RL1 Antibody (0902-2)

Immunogen

Synthetic peptide within mouse il1rl1 aa 20-50.

Host

Rabbit

Positive Control

D3 cell, Zebrafish tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-2,000

TARGET

UNIPROT #

PROTEIN NAME

Interleukin-1 receptor-like 1

GENE NAME

Il1rl1

SEQUENCE SIMILARITIES

Belongs to the interleukin-1 receptor family.

TISSUE SPECIFICITY

Predominantly expressed in hematopoietic tissues, and in macrophage, erythroid, epithelial and fibroblast cell lines. Isoform A is expressed in brain astrocytes and microglia. Isoform B is expressed in brain endothelial cells.

DEVELOPMENTAL STAGE

Expressed first in the fetal liver and then in lung and hematopoietic tissues.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type I membrane protein.; [Isoform B]: Secreted.

FUNCTION

Receptor for interleukin-33 (IL-33); signaling requires association of the coreceptor IL1RAP. Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8 (By similarity). Possibly involved in helper T-cell function.; [Isoform B]: Inhibits IL-33 signaling.

CITATIONS

  • Zhao, Guiyun et al.

    Cell surface proteomics analysis indicates a neural lineage bias of rat bone marrow mesenchymal stromal cells. | BioMed Research International [2014]