PRODUCT CODE: ER1803-31

IL12B Antibody (ER1803-31)

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

s of IL12B on different lysates using anti-IL12B antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Hela<br />
 Lane 2: Mouse placenta<br />
  • s of IL12B on different lysates using anti-IL12B antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Hela<br />
 Lane 2: Mouse placenta<br />
s of IL12B on different lysates using anti-IL12B antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Mouse placenta

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IL12B Antibody (ER1803-31)

Immunogen

Synthetic peptide within mouse il12b 220-300 aa.

Host

Rabbit

Positive Control

Hela, Mouse placenta tissue lysate.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

Interleukin-12 subunit beta

GENE NAME

IL12B

SYNONYMS

IL-12B, CLMF p40, NKSF2, IL12B, NKSF2

SEQUENCE SIMILARITIES

Belongs to the IL-12B family.

POST-TRANSLATIONAL MODIFICATION

Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.; Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.