PRODUCT CODE: ER1802-36

IL1 alpha Antibody (ER1802-36)

Applications

  • IHC-P

  • FC

  • WB

REACTIVITY

  • Human

  • Mouse

Western blot analysis of IL1 alpha on human lymph node lysates using anti-IL1 alpha antibody at 1/500 dilution.
  • Western blot analysis of IL1 alpha on human lymph node lysates using anti-IL1 alpha antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IL1 alpha antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of PC-3M cells with IL1 alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of IL1 alpha on human lymph node lysates using anti-IL1 alpha antibody at 1/500 dilution.

Applications

  • IHC-P

  • FC

  • WB

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IL1 alpha Antibody (ER1802-36)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human lymph node lysates, Human colon cancer tissue, PC-3M.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified

MOLECULAR WEIGHT

31 kDa

Isotype

IgG

APPLICATION DILUTION

  • IHC-P

  • 1:400-1:800

  • FC

  • 1:50-1:100

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

IL1 alpha

SYNONYMS

BAF antibody; FAF antibody; Hematopoietin 1 antibody; Hematopoietin-1 antibody; IL 1 alpha antibody; IL 1A antibody; IL-1 alpha antibody; Il-1a antibody; IL1 ALPHA antibody; IL1 antibody; IL1A antibody; IL1A_HUMAN antibody; IL1F1 antibody; Interleukin 1 alpha antibody; Interleukin-1 alpha antibody; Interleukin1 alpha antibody; LAF antibody; LEM antibody; Preinterleukin 1 alpha antibody; Pro interleukin 1 alpha antibody

SEQUENCE SIMILARITIES

Belongs to the IL-1 family.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Two forms of interleukin-1, designated IL-1α and IL-1β, have been described. Although encoded by distinct genes and exhibiting roughly only 25% sequence identity, IL-1α and IL-1β bind to the same receptor and seem to elicit similar biological responses. IL-1 production is generally thought to be associated with inflammation, but it has also been shown to be expressed during kidney development, thymocyte differentiation and cartilage degradation. IL-1 plays a critical role in the regulation of immune response and inflammation, acting as an activator of T and B lymphocytes and natural killer (NK) cells. In T cells, IL-1 stimulates the production of IL-2 and selectively inhibits IL-4 expression. IL-1 induces B cell proliferation and maturation, and immunoglobulin synthesis. NK cells require IL-1β for production of the anti- pathogen IFN-γ. IL-1 has also been implicated in several pathological conditions including rheumatoid arthritis, inflammatory bowel disease and atherosclerosis.