PRODUCT CODE: EM30301

IL-6 Monoclonal Antibody (EM30301)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of IL-6 on different cell lysates using anti-IL-6 antibody at 1/500 dilution.<br />
Positive control: <br />
Lane 1: Jurkat <br />
Lane 2: Raji <br />
Lane 3: Daudi
  • Western blot analysis of IL-6 on different cell lysates using anti-IL-6 antibody at 1/500 dilution.<br />
Positive control: <br />
Lane 1: Jurkat <br />
Lane 2: Raji <br />
Lane 3: Daudi
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-6 antibody. Counter stained with hematoxylin.
Western blot analysis of IL-6 on different cell lysates using anti-IL-6 antibody at 1/500 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Raji
Lane 3: Daudi

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

IL-6 Monoclonal Antibody (EM30301)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Jurkat, Raji, Daudi, human tonsil tissue

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

7-A7

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

24 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Interleukin-6

GENE NAME

Il6

SYNONYMS

IL-6

SEQUENCE SIMILARITIES

Belongs to the IL-6 superfamily.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.