PRODUCT CODE: EM1701-46

IFNAR1 Monoclonal Antibody (EM1701-46)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of IFNAR1 on different lysates using anti- IFNAR1 antibody at 1/500 dilution. <br />
  Positive control:<br />
  Lane 1: K562 <br />
  Lane 1: A431<br />
  Lane 1: Siha <br />
  Lane 1: Mouse brain tissue
  • Western blot analysis of IFNAR1 on different lysates using anti- IFNAR1 antibody at 1/500 dilution. <br />
  Positive control:<br />
  Lane 1: K562 <br />
  Lane 1: A431<br />
  Lane 1: Siha <br />
  Lane 1: Mouse brain tissue
  • ICC staining IFNAR1(red) in NCCIT cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IFNAR1(green) in SHG-44 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin.
Western blot analysis of IFNAR1 on different lysates using anti- IFNAR1 antibody at 1/500 dilution.
Positive control:
Lane 1: K562
Lane 1: A431
Lane 1: Siha
Lane 1: Mouse brain tissue

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

IFNAR1 Monoclonal Antibody (EM1701-46)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

K562, A431, Siha, mouse brain tissue lysate, NCCIT, SHUG-44, human lung tissue, human skin tissue, human colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A5-A3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

56 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

IFNAR1

SYNONYMS

Alpha type antiviral protein antibody; Antiviral protein, alpha-type antibody; Antiviral protein, beta-type antibody; AVP antibody; Beta type antiviral protein antibody; CRF2-1 antibody; Cytokine receptor class-II member 1 antibody; Cytokine receptor family 2 member 1 antibody; IFN alpha REC antibody; IFN alpha receptor antibody; IFN alpha/beta Receptor alpha antibody; IFN beta receptor antibody; IFN Interferon-beta receptor antibody; IFN-alpha/beta receptor 1 antibody; IFN-R-1 antibody; IFNAR antibody; Ifnar1 antibody; IFNBR antibody; IFRC antibody; INAR1_HUMAN antibody; Interferon (alpha beta and omega) receptor 1 antibody; interferon alpha and beta receptor subunit 1 antibody; Interferon alpha/beta receptor 1 antibody; Interferon alpha/beta receptor alpha chain antibody; Interferon beta receptor 1 antibody; interferon receptor 1 antibody; Interferon-alpha receptor antibody; Type I interferon receptor 1 antibody

SEQUENCE SIMILARITIES

Belongs to the type II cytokine receptor family.

TISSUE SPECIFICITY

IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated, leading to its internalization and degradation. Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation. The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex.; Phosphorylated on serine residues in response to interferon binding; this promotes interaction with FBXW11 and ubiquitination. Phosphorylated on tyrosine residues by TYK2 tyrosine kinase. Phosphorylated on tyrosine residues in response to interferon.; Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2.

SUBCELLULAR LOCATION

Endosome. Cell membrane.

FUNCTION

Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1. Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves. Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway.