PRODUCT CODE: ER2001-46

IFITM1 Antibody (ER2001-46)

Applications

  • WB

REACTIVITY

  • Human

Western blot analysis of IFITM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control:<br />
 Lane 1: A172 cell lysate<br />
 Lane 2: K562 cell lysate
  • Western blot analysis of IFITM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control:<br />
 Lane 1: A172 cell lysate<br />
 Lane 2: K562 cell lysate
Western blot analysis of IFITM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A172 cell lysate
Lane 2: K562 cell lysate

Applications

  • WB

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IFITM1 Antibody (ER2001-46)

Immunogen

Synthetic peptide within human ifitm1 aa 1-50.

Host

Rabbit

Positive Control

A172 cell lysate, K562 cell lysate.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

14 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

IFITM1

SYNONYMS

9-27 antibody; CD 225 antibody; CD 225 antigen antibody; CD225 antibody; CD225 antigen antibody; Dispanin subfamily A member 2a antibody; DSPA2a antibody; IFI 17 antibody; IFI17 antibody; IFITM1 antibody; IFM1_HUMAN antibody; Interferon induced protein 17 antibody; interferon induced transmembrane protein 1 (9-27) antibody; Interferon induced transmembrane protein 1 antibody; Interferon inducible protein 9-27 antibody; Interferon-induced protein 17 antibody; Interferon-induced transmembrane protein 1 antibody; Interferon-inducible protein 9-27 antibody; Leu 13 antibody; Leu 13 antigen antibody; Leu-13 antigen antibody; LEU13 antibody

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV S protein-mediated viral entry. Also implicated in cell adhesion and control of cell growth and migration. Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activation or by arresting cell growth in G1 phase in a p53-dependent manner. Acts as a positive regulator of osteoblast differentiation.