PRODUCT CODE: ER1706-94

IDO Antibody (ER1706-94)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of IDO on human placenta tissue lysate using anti-IDO antibody at 1/500 dilution. <br />
  • Western blot analysis of IDO on human placenta tissue lysate using anti-IDO antibody at 1/500 dilution. <br />
  • ICC staining IDO in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining IDO in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  •  Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IDO antibody. Counter stained with hematoxylin.
  •  Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IDO antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HUVEC cells with IDO antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of IDO on human placenta tissue lysate using anti-IDO antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

IDO Antibody (ER1706-94)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human placenta tissue lysate, HUVEC, Hela, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified

MOLECULAR WEIGHT

45 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Indoleamine 2,3-dioxygenase 1

GENE NAME

IDO1

SYNONYMS

IDO-1, IDO1, IDO, INDO

SEQUENCE SIMILARITIES

Belongs to the indoleamine 2,3-dioxygenase family.

TISSUE SPECIFICITY

Expressed in mature dendritic cells located in lymphoid organs (including lymph nodes, spleen, tonsils, Peyers's patches, the gut lamina propria, and the thymic medulla), in some epithelial cells of the female genital tract, as well as in endothelial cells of term placenta and in lung parenchyma. Weakly or not expressed in most normal tissues, but mostly inducible in most tissues. Expressed in more than 50% of tumors, either by tumoral, stromal, or endothelial cells (expression in tumor is associated with a worse clinical outcome). Not overexpressed in tumor-draining lymph nodes.

SUBCELLULAR LOCATION

Cytoplasm, cytosol.

FUNCTION

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity. Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection.