PRODUCT CODE: ER131207

ICAM1 Antibody (ER131207)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of ICAM1 on different cell lysates using anti- ICAM1 antibody at 1/500 dilution.<br />
 Positive control: <br />
  Lane 1: Raji <br />
  Lane 2: HUVEC <br />
  Lane 3: K562
  • Western blot analysis of ICAM1 on different cell lysates using anti- ICAM1 antibody at 1/500 dilution.<br />
 Positive control: <br />
  Lane 1: Raji <br />
  Lane 2: HUVEC <br />
  Lane 3: K562
  • ICC staining of ICAM1 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with ICAM1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of ICAM1 on different cell lysates using anti- ICAM1 antibody at 1/500 dilution.
Positive control:
Lane 1: Raji
Lane 2: HUVEC
Lane 3: K562

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ICAM1 Antibody (ER131207)

Immunogen

Peptide

Host

Rabbit

Positive Control

Raji, HUVEC, K562, human tonsil tissue, Hela

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

90 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

ICAM1

SYNONYMS

Antigen identified by monoclonal antibody; BB2 antibody; BB 2 antibody; BB2 antibody; CD 54 antibody; CD_antigen=CD54 antibody; CD54 antibody; Cell surface glycoprotein P3.58 antibody; Human rhinovirus receptor antibody; ICAM 1 antibody; ICAM-1 antibody; ICAM1 antibody; ICAM1_HUMAN antibody; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody; Intercellular adhesion molecule 1 antibody; Major group rhinovirus receptor antibody; MALA 2 antibody; MALA2 antibody; MyD 10 antibody; MyD10 antibody; P3.58 antibody; Surface antigen of activated B cells, BB2 antibody

SEQUENCE SIMILARITIES

Belongs to the immunoglobulin superfamily. ICAM family.

POST-TRANSLATIONAL MODIFICATION

Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

SUBCELLULAR LOCATION

Cell membrane, Nucleus, Mitochondrion

FUNCTION

ICAM-1 is a member of the immunoglobulin superfamily, the superfamily of proteins including antibodies and T-cell receptors. The structure of ICAM-1 is characterized by heavy glycosylation, and the protein’s extracellular domain is composed of multiple loops created by disulfide bridges within the protein. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor (TNF) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. More recently, ICAM-1 has been characterized as a site for the cellular entry of human rhinovirus. ICAM-1 and soluble ICAM-1 have antagonistic effects on the tight junctions forming the blood-testis barrier, thus playing a major role in spermatogenesis. ICAM-1 has been implicated in subarachnoid hemorrhage (SAH).