PRODUCT CODE: EM21103

HSP90 Beta Monoclonal Antibody (EM21103)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of HSP90 beta on different cell lysates using anti- HSP90 beta antibody at 1/5000 dilution.<br />
 Positive control: <br />
  Lane 1: Hela <br />
  Lane 2: NIH/3T3 <br />
  Lane 3: PC12 <br />
  Lane 4: 293T <br />
  Lane 5: HePG2 <br />
  Lane 6: COS-1 <br />
  Lane 7: Jurkat <br />
  Lane 8: A549 <br />
  Lane 9: Raji <br />
  Lane 10: Mouse heart tissue
  • Western blot analysis of HSP90 beta on different cell lysates using anti- HSP90 beta antibody at 1/5000 dilution.<br />
 Positive control: <br />
  Lane 1: Hela <br />
  Lane 2: NIH/3T3 <br />
  Lane 3: PC12 <br />
  Lane 4: 293T <br />
  Lane 5: HePG2 <br />
  Lane 6: COS-1 <br />
  Lane 7: Jurkat <br />
  Lane 8: A549 <br />
  Lane 9: Raji <br />
  Lane 10: Mouse heart tissue
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.
Western blot analysis of HSP90 beta on different cell lysates using anti- HSP90 beta antibody at 1/5000 dilution.
Positive control:
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: PC12
Lane 4: 293T
Lane 5: HePG2
Lane 6: COS-1
Lane 7: Jurkat
Lane 8: A549
Lane 9: Raji
Lane 10: Mouse heart tissue

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

HSP90 Beta Monoclonal Antibody (EM21103)

Immunogen

Peptide

Host

Mouse

Positive Control

Hela, NIH/3T3, PC12, 293T, A431, COS-1, Jurkat, HepG2, A549, Raji, mouse brain tissue, mouse heart tissue, rat brain tissue , rat heart tissue,human colon carcinoma tissue,human tonsil tissue,mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

2-1-G3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

84 kDa

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:5,000

  • IHC-P

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

HSP90 Beta

SYNONYMS

90 kda heat shock protein beta HSP90 beta antibody; D6S182 antibody; FLJ26984 antibody; Heat shock 84 kDa antibody; Heat shock 90kD protein 1, beta antibody; Heat shock 90kDa protein 1 beta antibody; Heat shock protein 90 alpha family class B member 1 antibody; Heat shock protein 90 kDa antibody; Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody; Heat shock protein 90kDa alpha family class B member 1 antibody; Heat shock protein beta antibody; Heat shock protein HSP 90 beta antibody; Heat shock protein HSP 90-beta antibody; HS90B_HUMAN antibody; HSP 84 antibody; HSP 90 antibody; HSP 90 b antibody; HSP 90b antibody; HSP84 antibody; HSP90 BETA antibody; hsp90ab1 antibody; HSP90B antibody; HSPC2 antibody; HSPCB antibody

SEQUENCE SIMILARITIES

Belongs to the heat shock protein 90 family.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).; ISGylated.; S-nitrosylated; negatively regulates the ATPase activity.; Phosphorylation at Tyr-301 by SRC is induced by lipopolysaccharide. Phosphorylation at Ser-226 and Ser-255 inhibits AHR interaction.; Methylated by SMYD2; facilitates dimerization and chaperone complex formation; promotes cancer cell proliferation.; Cleaved following oxidative stress resulting in HSP90AB1 protein radicals formation; disrupts the chaperoning function and the degradation of its client proteins.

SUBCELLULAR LOCATION

Cytoplasm

FUNCTION

Hsp90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress. Two isoforms which correspond to the major and minor isoform , (Hsp90 α and Hsp90 β ) can be found in nearly equal amount in humans, and operates as part of a multichaperone machinery in the cytosol, which includes Hsp70, peptidyl-prolyl isomerases and other cochaperones.