PRODUCT CODE: ER31216

HSP27 Antibody (ER31216)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Hsp27 on different cell lysates using anti- Hsp27 antibody at 1/500 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: Jurkat <br />
 Lane 3: 293 <br />
 Lane 4: Human kidney <br />
 Lane 5: Mouse kidney <br />
 Lane 6: Mouse heart
  • Western blot analysis of Hsp27 on different cell lysates using anti- Hsp27 antibody at 1/500 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: Jurkat <br />
 Lane 3: 293 <br />
 Lane 4: Human kidney <br />
 Lane 5: Mouse kidney <br />
 Lane 6: Mouse heart
  • ICC staining Hsp27 in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Hsp27 in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Hsp27 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Hsp27 antibody. Counter stained with hematoxylin.
Western blot analysis of Hsp27 on different cell lysates using anti- Hsp27 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: 293
Lane 4: Human kidney
Lane 5: Mouse kidney
Lane 6: Mouse heart

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

HSP27 Antibody (ER31216)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, Jurkat, 293, mouse kidney tissue, human kidney tissue, mouse heart tissue,A172,Lovo,human colon cancer tissue, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

27 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:100

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

HSP27

SYNONYMS

Heat shock 27kDa protein antibody; 28 kDa heat shock protein antibody; CMT2F antibody; DKFZp586P1322 antibody; epididymis secretory protein Li 102 antibody; Estrogen regulated 24 kDa protein antibody; Estrogen-regulated 24 kDa protein antibody; Heat shock 25kDa protein 1 antibody; Heat shock 27 kDa protein antibody; Heat shock 27kD protein 1 antibody; Heat shock 27kDa protein 1 antibody; Heat shock 28kDa protein 1 antibody; Heat Shock Protein 27 antibody; Heat shock protein beta 1 antibody; Heat shock protein beta-1 antibody; heat shock protein family B (small) member 1 antibody; HEL-S-102 antibody; HMN2B antibody; HS.76067 antibody; Hsp 25 antibody; HSP 27 antibody; Hsp 28 antibody; Hsp B1 antibody; Hsp25 antibody; HSP27 antibody; Hsp28 antibody; HspB1 antibody; HSPB1_HUMAN antibody; SRP27 antibody; Stress responsive protein 27 antibody; Stress-responsive protein 27 antibody

SEQUENCE SIMILARITIES

Belongs to the small heat shock protein (HSP20) family.

TISSUE SPECIFICITY

Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement.

SUBCELLULAR LOCATION

Cytoplasm, nucleus

FUNCTION

Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, α-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. Hsp27 appears in many cell types, especially all types of muscle cells. It is located mainly in the cytosol, but also in the perinuclear region, endoplasmatic reticulum, and nucleus. It is overexpressed during different stages of cell differentiation and development. This suggests an essential role for Hsp27 in the differentiation of tissues. Probably Hsp27 – among other chaperones – is involved in the process of cell differentiation. Changes of Hsp27 levels were observed in Ehrlich ascite cells, embryonic stem cells, normal B-cells, B-lymphoma cells, osteoblasts, keratinocytes, neurons etc.

CITATIONS

  • Li, Jiadai et al.

    Comparative Transcriptome Analysis Reveals Different Host Cell Responses to Acute and Persistent Foot-and-Mouth Disease Virus Infection. | Virologica Sinica [2020]