PRODUCT CODE: em1701-52

HNP-1 Monoclonal Antibody (EM1701-52)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

Western blot analysis of HNP-1 on rat spleen tissue lysates using anti-HNP-1 antibody at 1/500 dilution.<br />
  • Western blot analysis of HNP-1 on rat spleen tissue lysates using anti-HNP-1 antibody at 1/500 dilution.<br />
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HNP-1 antibody. Counter stained with hematoxylin.
Western blot analysis of HNP-1 on rat spleen tissue lysates using anti-HNP-1 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

HNP-1 Monoclonal Antibody (EM1701-52)

Immunogen

Peptide

Host

Mouse

Positive Control

Rat spleen tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

2D2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

10 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Neutrophil defensin 1

GENE NAME

DEFA1; DEFA1B

SYNONYMS

HP-1, HP1, HP-2, HP2, DEFA1, DEF1, DEFA2, MRS

SEQUENCE SIMILARITIES

Belongs to the alpha-defensin family.

POST-TRANSLATIONAL MODIFICATION

ADP-ribosylation drastically reduces cytotoxic and antibacterial activities, and enhances IL8 production.; Phosphorylation at Tyr-85 has been found in some cancer cell lines, and interferes with ADP-ribosylation.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.