PRODUCT CODE: ER1802-52

Hip1 Antibody (ER1802-52)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Hip1 on different tissue lysates using anti-Hip1 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse testis <br />
  Lane 2: Mouse spinal cord <br />
  Lane 3: SH-SY5Y
  • Western blot analysis of Hip1 on different tissue lysates using anti-Hip1 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse testis <br />
  Lane 2: Mouse spinal cord <br />
  Lane 3: SH-SY5Y
  • ICC staining Hip1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Hip1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Hip1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Hip1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hip1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-Hip1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with Hip1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Hip1 on different tissue lysates using anti-Hip1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse testis
Lane 2: Mouse spinal cord
Lane 3: SH-SY5Y

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Hip1 Antibody (ER1802-52)

Immunogen

Synthetic peptide within c terminal human hip1.

Host

Rabbit

Positive Control

Mouse testis tissue lysate, Mouse spinal cord tissue lysate, A549, PC-3M, SH-SY5Y, human colon cancer tissue, mouse brain tissue, mouse fallopian tubes tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

116 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Hip1

SYNONYMS

2610109B09Rik antibody; A930014B11Rik antibody; E130315I21Rik antibody; HIP 1 antibody; HIP I antibody; HIP-1 antibody; HIP-I antibody; hip1 antibody; HIP1/PDGFRB fusion gene antibody; HIP1/PDGFRB fusion gene, included antibody; HIP1_HUMAN antibody; HIPI antibody; Huntingtin interacting protein 1 antibody; Huntingtin-interacting protein 1 antibody; Huntingtin-interacting protein I antibody; ILWEQ antibody; KIAA4113 antibody; MGC126506 antibody; MGC27616 antibody; mKIAA4113 antibody

SEQUENCE SIMILARITIES

Belongs to the SLA2 family.

TISSUE SPECIFICITY

Ubiquitously expressed with the highest level in brain. Expression is up-regulated in prostate and colon cancer.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

Huntington disease is associated with the expansion of a polyglutamine tract, greater than 35 repeats, in the HD gene product huntingtin. HIP1 (huntingtin-interacting protein 1), a membrane-associated protein, binds specifically to the N-terminus of human huntingtin. HIP1 is ubiquitously expressed in different brain regions at low levels, and exhibits nearly identical subcellular fractionation as huntingtin. The huntingtin-HIP1 interaction is restricted to the brain and is inversely correlated to the polyglutamine length in the huntingtin, suggesting that loss of normal huntingtin-HIP1 interaction may compromise the membrane-cytoskeletal integrity in the brain. HIP1 contains an endocytic multidomain protein with a C-terminal Actin-binding domain, a central coiled-coil forming region and an N-terminal ENTH domain. HIP1 may be involved in vesicle trafficking; the structural integrity of HIP1 is crucial for maintenance of normal vesicle size in vivo. HIP12 is a non-proapoptotic member of the HIP gene family that is expressed in the brain and shares a similar subcellular distribution pattern with HIP1. However, HIP12 differs from HIP1 in its pattern of expression at both the mRNA and protein level. HIP12 does not directly interact with huntingtin but can interact with HIP1.