PRODUCT CODE: ER0106

HER2 / ErbB2 Antibody (ER0106)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of ErbB2 on different cell lysates using anti- ErbB2 antibody at 1/500 dilution.<br />
Positive control: <br />
 Lane 1: SKOV-3 <br />
 Lane 2: SKBR-3
  • Western blot analysis of ErbB2 on different cell lysates using anti- ErbB2 antibody at 1/500 dilution.<br />
Positive control: <br />
 Lane 1: SKOV-3 <br />
 Lane 2: SKBR-3
  • ICC staining ErbB2 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Her 2 antibody. Counter stained with hematoxylin.
Western blot analysis of ErbB2 on different cell lysates using anti- ErbB2 antibody at 1/500 dilution.
Positive control:
Lane 1: SKOV-3
Lane 2: SKBR-3

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

HER2 / ErbB2 Antibody (ER0106)

Immunogen

Peptide

Host

Rabbit

Positive Control

SKOV-3, SKBR-3, human stomach carcinoma tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

140kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Receptor tyrosine-protein kinase erbB-2

GENE NAME

ERBB2

SYNONYMS

MLN 19

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.

TISSUE SPECIFICITY

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues. Signaling via SEMA4C promotes phosphorylation at Tyr-1248. Dephosphorylated by PTPN12.

SUBCELLULAR LOCATION

[Isoform 1]: Cell membrane; Single-pass type I membrane protein. Early endosome. Cytoplasm, perinuclear region. Nucleus. Note=Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. Also detected in VPS35-positive endosome-to-TGN retrograde vesicles.; [Isoform 2]: Cytoplasm. Nucleus.; [Isoform 3]: Cytoplasm. Nucleus.

FUNCTION

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.; In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.