PRODUCT CODE: ER1802-73

Heme Oxygenase 1 (HO-1) Antibody (ER1802-73)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Heme Oxygenase 1 (HO-1) on mouse spleen tissue lysate using anti-Heme Oxygenase 1 (HO-1) antibody at 1/500 dilution.
  • Western blot analysis of Heme Oxygenase 1 (HO-1) on mouse spleen tissue lysate using anti-Heme Oxygenase 1 (HO-1) antibody at 1/500 dilution.
  • ICC staining Heme Oxygenase 1 (HO-1) in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Heme Oxygenase 1 (HO-1) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.
Western blot analysis of Heme Oxygenase 1 (HO-1) on mouse spleen tissue lysate using anti-Heme Oxygenase 1 (HO-1) antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Heme Oxygenase 1 (HO-1) Antibody (ER1802-73)

Immunogen

Synthetic peptide of n-terminal human heme oxygenase 1 (ho-1).

Host

Rabbit

Positive Control

Mouse spleen tissue lysate, 293T, HepG2, human spleen tissue, human placenta tissue, human small intestine tissue, mouse small intestine tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

33 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Heme oxygenase 1

GENE NAME

HMOX1

SYNONYMS

HO-1, HMOX1, HO, HO1

SEQUENCE SIMILARITIES

Belongs to the heme oxygenase family.

TISSUE SPECIFICITY

Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).

SUBCELLULAR LOCATION

Microsome. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.

FUNCTION

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.