PRODUCT CODE: ER1901-56

HDG11 Antibody (ER1901-56)

Applications

  • WB

  • IHC-P

  • ELISA

REACTIVITY

  • Arabidopsis thaliana

Western blot analysis of HDG11 on HDG11 recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-56) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: HDG11 recombinant protein with primary antibody at 1:200 dilutions<br />
 Lane 2: HDG11 recombinant protein with primary antibody at 1:1,000 dilutions
  • Western blot analysis of HDG11 on HDG11 recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-56) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: HDG11 recombinant protein with primary antibody at 1:200 dilutions<br />
 Lane 2: HDG11 recombinant protein with primary antibody at 1:1,000 dilutions
  • Immunohistochemical analysis of paraffin-embedded A. thaliana<br />
  tissue using anti-HDG11 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of HDG11 on HDG11 recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-56) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HDG11 recombinant protein with primary antibody at 1:200 dilutions
Lane 2: HDG11 recombinant protein with primary antibody at 1:1,000 dilutions

Applications

  • WB

  • IHC-P

  • ELISA

REACTIVITY

  • Arabidopsis thaliana

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

HDG11 Antibody (ER1901-56)

Immunogen

Synthetic peptide within a. thaliana hdg11 aa 620-690.

Host

Rabbit

Positive Control

A. thaliana tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

79 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:2,000

  • IHC-P:1:50-1:200

  • ELISA:1:250-1:256,000

TARGET

UNIPROT #

PROTEIN NAME

HDG11

SYNONYMS

Homeobox-leucine zipper protein HDG11; HD-ZIP protein HDG11; Homeodomain GLABRA 2-like protein 11; Homeodomain transcription factor HDG11; Protein ENHANCED DROUGHT TOLERANCE 1; Protein HOMEODOMAIN GLABROUS 11; HDG11; EDT1; HDGL2-11; At1g73360; T9L24.43

SEQUENCE SIMILARITIES

Belongs to the HD-ZIP homeobox family. Class IV subfamily.

TISSUE SPECIFICITY

Expressed in apical meristems and young epidermal tissue including trichomes and stipules. Expressed in lateral root tips, the L1 layer of apical inflorescence meristems and early flower primordia, carpel and petal epidermis, stigma papillae, ovule primordia, nucellus and embryo.

DEVELOPMENTAL STAGE

During embryo development, expressed in all cells at the 4- and 16-cell embryo stages. Expression is restricted to the protoderm from the globular stage onward.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Transcription factor which acts as positive regulator of drought stress tolerance. Can transactivate CIPK3, NCED3 and ERECTA. Transactivates several cell-wall-loosening protein genes by directly binding to HD motifs in their promoters. These target genes play important roles in coordinating cell-wall extensibility with root development and growth. Transactivates CYP74A/AOS, AOC3, OPR3 and 4CLL5/OPCL1 genes by directly binding to HD motifs in their promoters. These target genes are involved in jasmonate (JA) biosynthesis, and JA signaling affects root architecture by activating auxin signaling, which promotes lateral root formation. Acts as negative regulator of trichome branching. Required for the establishment of giant cell identity on the abaxial side of sepals. May regulate cell differentiation and proliferation during root and shoot meristem development.