HADHSC Recombinant Mouse Monoclonal Antibody [D10-E7-R] (HA601203)
Catalog# HA601203
Mouse Monoclonal to HADHSC
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of HADH on different lysates with Mouse anti-HADH antibody (HA601203) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: HT-29 cell lysate (20 µg/Lane)
Lane 3: HL-60 cell lysate (20 µg/Lane)
Lane 4: A431 cell lysate (20 µg/Lane)
Lane 5: K-562 cell lysate (20 µg/Lane)
Lane 6: Human liver tissue lysate (40 µg/Lane)
Lane 7: Mouse liver tissue lysate (40 µg/Lane)
Lane 8: Mouse heart tissue lysate (40 µg/Lane)
Lane 9: Rat liver tissue lysate (40 µg/Lane)
Lane 10: Rat heart tissue lysate (40 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601203) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: HT-29 cell lysate (20 µg/Lane)
Lane 3: HL-60 cell lysate (20 µg/Lane)
Lane 4: A431 cell lysate (20 µg/Lane)
Lane 5: K-562 cell lysate (20 µg/Lane)
Lane 6: Human liver tissue lysate (40 µg/Lane)
Lane 7: Mouse liver tissue lysate (40 µg/Lane)
Lane 8: Mouse heart tissue lysate (40 µg/Lane)
Lane 9: Rat liver tissue lysate (40 µg/Lane)
Lane 10: Rat heart tissue lysate (40 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601203) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.