PRODUCT CODE: ER1802-63

GST3 Antibody (ER1802-63)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of GST3 on different cell lysate using anti-GST3 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: Siha <br />
  Lane 2: Hela<br />
  Lane 3: A549 <br />
  Lane 4: Mouse kidney tissue<br />
  Lane 5: Rat liver tissue
  • Western blot analysis of GST3 on different cell lysate using anti-GST3 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: Siha <br />
  Lane 2: Hela<br />
  Lane 3: A549 <br />
  Lane 4: Mouse kidney tissue<br />
  Lane 5: Rat liver tissue
  • ICC staining GST3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GST3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GST3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-GST3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GST3 antibody. Counter stained with hematoxylin.
Western blot analysis of GST3 on different cell lysate using anti-GST3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Siha
Lane 2: Hela
Lane 3: A549
Lane 4: Mouse kidney tissue
Lane 5: Rat liver tissue

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

GST3 Antibody (ER1802-63)

Immunogen

Recombinant protein with human gst3 aa 1-150.

Host

Rabbit

Positive Control

Siha, Hela, A549, mouse kidney tissue lysate, rat liver tissue lysate, HUVEC, LOVO, human liver cancer tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

23 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

GST3

SYNONYMS

Deafness antibody; Deafness X-linked 7 antibody; DFN7 antibody; FAEES3 antibody; Fatty Acid Ethyl Ester Synthase III antibody; Glutathione S Transferase 3 antibody; Glutathione S Transferase Pi antibody; Glutathione S-transferase P antibody; Glutathione S-transferase pi 1 antibody; GST class-pi antibody; GST3 antibody; GSTP antibody; Gstp1 antibody; GSTP1-1 antibody; GSTP1_HUMAN antibody; PI antibody; X linked 7 antibody

SEQUENCE SIMILARITIES

Belongs to the GST superfamily. Pi family.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Mitochondrion.

FUNCTION

Glutathione S-transferases (GSTs) function in the metabolic detoxification of various environmental carcinogens and lipid hydroperoxides. In response to oxidative stress, upregulation of the GST family member GSTP1 occurs, consistent with this function. Furthermore, the GSTP1 gene is subject to CpG island hypermethylation, a state that correlates with human prostatic carcinogenesis. GSTP1 gene hypermethylation can be detected in urine, ejaculate and plasma from men with prostate cancer, potentially making GSTP1 a useful biomarker for prostate cancer screening.