PRODUCT CODE: ER1511-5

GRP94 Antibody (ER1511-5)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of GRP94 on different cell lysate using anti-GRP94 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: HepG2 <br />
 Lane 2: NIH-3T3<br />
 Lane 3: PC-12<br />
  • Western blot analysis of GRP94 on different cell lysate using anti-GRP94 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: HepG2 <br />
 Lane 2: NIH-3T3<br />
 Lane 3: PC-12<br />
  • ICC staining GRP94 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GRP94 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GRP94 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
Western blot analysis of GRP94 on different cell lysate using anti-GRP94 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: NIH-3T3
Lane 3: PC-12

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

GRP94 Antibody (ER1511-5)

Immunogen

Peptide.

Host

Rabbit

Positive Control

HepG2, NIH-3T3, PC-12, Hela, MCF-7, human liver tissue, human stomach cancer, human breast tissue, mouse stomach tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

94 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Endoplasmin

GENE NAME

HSP90B1

SYNONYMS

GRP-94, HSP90B1, GRP94, TRA1

SEQUENCE SIMILARITIES

Belongs to the heat shock protein 90 family.

SUBCELLULAR LOCATION

Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

FUNCTION

Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity (By similarity).