PRODUCT CODE: ER40402

GRP78 Antibody (ER40402)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of GRP78 on different cell lysates using anti-GRP78 antibody at 1/2000 dilution.
  • Western blot analysis of GRP78 on different cell lysates using anti-GRP78 antibody at 1/2000 dilution.
  • ICC staining GRP78 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GRP78 in SK-BR-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GRP78 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • IF staining GRP78 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
  • Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
Western blot analysis of GRP78 on different cell lysates using anti-GRP78 antibody at 1/2000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

GRP78 Antibody (ER40402)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, PC12, HepG2, SK-BR-3, human liver tissue, rat epencephalon tissue, mouse liver tissue, rat small intestine tissue, mouse small intestine, human colon cancer tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

72 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

GRP78

SYNONYMS

78 kDa glucose regulated protein antibody; 78 kDa glucose-regulated protein antibody; AL022860 antibody; AU019543 antibody; BIP antibody; D2Wsu141e antibody; D2Wsu17e antibody; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody; Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody; Epididymis secretory sperm binding protein Li 89n antibody; FLJ26106 antibody; Glucose Regulated Protein 78kDa antibody; GRP 78 antibody; GRP-78 antibody; GRP78 antibody; GRP78_HUMAN antibody; Heat shock 70 kDa protein 5 antibody; Heat Shock 70kDa Protein 5 antibody; Heat shock protein family A (Hsp70) member 5 antibody; HEL S 89n antibody; Hsce70 antibody; HSPA 5 antibody; HSPA5 antibody; Immunoglobulin Heavy Chain Binding Protein antibody; Immunoglobulin heavy chain-binding protein antibody; mBiP antibody; MIF2 antibody; Sez7 antibody

SEQUENCE SIMILARITIES

Belongs to the heat shock protein 70 family.

POST-TRANSLATIONAL MODIFICATION

AMPylated by FICD. In unstressed cells, AMPylation at Thr-518 by FICD inactivates the chaperome activity: AMPylated form is locked in a relatively inert state and only weakly stimulated by J domain-containing proteins (By similarity). In response to endoplasmic reticulum stress, de-AMPylation by the same protein, FICD, restores the chaperone activity (By similarity).

SUBCELLULAR LOCATION

Cytoplasm, endoplasmic reticulum lumen

FUNCTION

Binding immunoglobulin protein (BiP) also known as 78 kDa glucose-regulated protein (GRP-78) or heat shock 70 kDa protein 5 (HSPA5) is a protein that in humans is encoded by the HSPA5 gene. BiP is a HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER) that binds newly synthesized proteins as they are translocated into the ER, and maintains them in a state competent for subsequent folding and oligomerization. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. Like many stress and heat shock proteins, BiP/GRP78 has potent immunological activity when released from the internal environment of the cell into the extracelluar space.specifically, it feeds anti-inflammatory and pro-resolutory signals into immune networks, thus helping to resolve inflammation.

CITATIONS

  • Li, Zhengzheng et al.

    Lipofectamine 2000/siRNA complexes cause endoplasmic reticulum unfolded protein response in human endothelial cells. | Journal of Cellular Physiology [2019]

  • Li, Heyangzi et al.

    Mitochondrial Transfer from Bone Marrow Mesenchymal Stem Cells to Motor Neurons in Spinal Cord Injury Rats via Gap Junction. | Theranostics [2019]

  • Tan, Zhouke et al.

    Pharmacological and genetic inhibition of fatty acid-binding protein 4 alleviated cisplatin-induced acute kidney injury. | Journal of Cellular and Molecular Medicine [2019]

  • Hao, Yan et al.

    2-Methylquinazoline derivative 23BB as a highly selective histone deacetylase 6 inhibitor alleviated cisplatin-induced acute kidney injury. | Bioscience Reports [2020]

  • Lin, Yinuo et al.

    Exosomes derived from HeLa cells break down vascular integrity by triggering endoplasmic reticulum stress in endothelial cells. | Journal of Extracellular Vesicles [2020]

  • Huang, Zhuo et al.

    Activation of GPR120 by TUG891 ameliorated cisplatin-induced acute kidney injury via repressing ER stress and apoptosis. | Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie [2020]