PRODUCT CODE: ER1706-50

GRP78 Antibody (ER1706-50)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of GRP78 on different lysates using anti-GRP78 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse liver tissue<br />
  Lane 2: Rat liver tissue<br />
  Lane 3: HepG2
  • Western blot analysis of GRP78 on different lysates using anti-GRP78 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse liver tissue<br />
  Lane 2: Rat liver tissue<br />
  Lane 3: HepG2
  • ICC staining GRP78 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GRP78 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GRP78 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with GRP78 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of GRP78 on different lysates using anti-GRP78 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse liver tissue
Lane 2: Rat liver tissue
Lane 3: HepG2

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

GRP78 Antibody (ER1706-50)

Immunogen

Peptide.

Host

Rabbit

Positive Control

Mouse liver tissue lysate, rat liver tissue lysate, HepG2, A431, HUVEC, rat brain tissue, human liver tissue, human placenta tissue, human stomach cancer tissue, mouse cerebellum tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

72 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

GRP78

SYNONYMS

78 kDa glucose regulated protein antibody; 78 kDa glucose-regulated protein antibody; AL022860 antibody; AU019543 antibody; BIP antibody; D2Wsu141e antibody; D2Wsu17e antibody; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody; Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody; Epididymis secretory sperm binding protein Li 89n antibody; FLJ26106 antibody; Glucose Regulated Protein 78kDa antibody; GRP 78 antibody; GRP-78 antibody; GRP78 antibody; GRP78_HUMAN antibody; Heat shock 70 kDa protein 5 antibody; Heat Shock 70kDa Protein 5 antibody; Heat shock protein family A (Hsp70) member 5 antibody; HEL S 89n antibody; Hsce70 antibody; HSPA 5 antibody; HSPA5 antibody; Immunoglobulin Heavy Chain Binding Protein antibody; Immunoglobulin heavy chain-binding protein antibody; mBiP antibody; MIF2 antibody; Sez7 antibody

SEQUENCE SIMILARITIES

Belongs to the heat shock protein 70 family.

POST-TRANSLATIONAL MODIFICATION

AMPylated by FICD. In unstressed cells, AMPylation at Thr-518 by FICD inactivates the chaperome activity: AMPylated form is locked in a relatively inert state and only weakly stimulated by J domain-containing proteins (By similarity). In response to endoplasmic reticulum stress, de-AMPylation by the same protein, FICD, restores the chaperone activity (By similarity).

SUBCELLULAR LOCATION

Endoplasmic reticulum. Cytoplasm.

FUNCTION

Plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate (By similarity). GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation.