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PRODUCT CODE: ER2001-72

GRK1 Antibody (ER2001-72)

Applications

  • WB

  • IHC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of GRK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: Mouse marrow tissue lysate<br /> Lane 2: HUVEC cell lysate<br /> Lane 3: 293 cell lysate<br /> Lane 4: Rat marrow tissue lysate
  • Western blot analysis of GRK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: Mouse marrow tissue lysate<br /> Lane 2: HUVEC cell lysate<br /> Lane 3: 293 cell lysate<br /> Lane 4: Rat marrow tissue lysate
  • Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue using anti-GRK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-72, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded rat eyeball tissue using anti-GRK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-72, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of GRK1 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-72, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of GRK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse marrow tissue lysate
Lane 2: HUVEC cell lysate
Lane 3: 293 cell lysate
Lane 4: Rat marrow tissue lysate

Applications

  • WB

  • IHC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

GRK1 Antibody (ER2001-72)

Immunogen

Synthetic peptide within human grk1 aa 500-563.

Host

Rabbit

Positive Control

Mouse marrow tissue lysate, HUVEC cell lysate, 293 cell lysate, rat marrow tissue lysate, mouse eyeball tissue, rat eyeball tissue, SHSY5Y.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

Predicted band size: 63 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC

  • 1:100-1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q15835 Human, Q9WVL4 Mouse, Q63651 Rat

PROTEIN NAME

Rhodopsin kinase GRK1 RK G protein-coupled receptor kinase 1

SUBCELLULAR LOCATION

Cell projection, Membrane.

FUNCTION

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2).