PRODUCT CODE: ER31203

GRB2 Antibody (ER31203)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of GRB2 on different cell lysates using anti-GRB2 antibody at 1/500 dilution.
  • Western blot analysis of GRB2 on different cell lysates using anti-GRB2 antibody at 1/500 dilution.
  • ICC staining GRB2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GRB2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-GRB2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SHG-44 cells with GRB2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of GRB2 on different cell lysates using anti-GRB2 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

GRB2 Antibody (ER31203)

Immunogen

Peptide

Host

Rabbit

Positive Control

F9, HepG2, SHG-44, human breast cancer tissue, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

25 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

GRB2

SYNONYMS

Abundant SRC homology antibody; Adapter protein GRB2 antibody; ASH antibody; Ash protein antibody; EGFRBP GRB2 antibody; Epidermal growth factor receptor binding protein antibody; Epidermal growth factor receptor binding protein GRB2 antibody; GRB 2 antibody; GRB2 adapter protein antibody; Grb2 antibody; GRB2_HUMAN antibody; Grb3 3 antibody; Growth factor receptor bound protein 2 antibody; Growth factor receptor bound protein 3 antibody; Growth factor receptor-bound protein 2 antibody; HT027 antibody; MST084 antibody; MSTP084 antibody; NCKAP2 antibody; OTTHUMP00000166096 antibody; OTTHUMP00000166097 antibody; OTTHUMP00000166098 antibody; Protein ash antibody; SEM5 antibody; SH2/SH3 adapter GRB2 antibody

SEQUENCE SIMILARITIES

Belongs to the GRB2/sem-5/DRK family.

SUBCELLULAR LOCATION

Cytoplasm, nucleus.

FUNCTION

Grb2 is widely expressed and is essential for multiple cellular functions. Inhibition of Grb2 function impairs developmental processes in various organisms and blocks transformation and proliferation of various cell types, and so it is not surprising that a targeted gene disruption of Grb2 in mouse is lethal at an early embryonic stage. Grb2 is best known for its ability to link the epidermal growth factor receptor tyrosine kinase to the activation of Ras and its downstream kinases, ERK1,2. Grb2 is composed of an SH2 domain flanked on each side by an SH3 domain. Grb2 has two closely related proteins with similar domain organizations, Gads and Grap. Gads and Grap are expressed specifically in hematopoietic cells and function in the coordination of tyrosine kinase mediated signal transduction. The SH2 domain of Grb2 binds to phosphorylated tyrosine-containing peptides on receptors or scaffold proteins with a preference for pY-X-N-X, where X is generally a hydrophobic residue such as valine.