Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
GRB2 Antibody (ER31203)
Immunogen
Peptide
Host
Rabbit
Positive Control
F9, HepG2, SHG-44, human breast cancer tissue, human tonsil tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified
MOLECULAR WEIGHT
25 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500
-
ICC
-
1:200
-
IHC-P
-
1:200
-
FC
-
1:100-1:200
TARGET
UNIPROT #
PROTEIN NAME
GRB2
SYNONYMS
Abundant SRC homology antibody; Adapter protein GRB2 antibody; ASH antibody; Ash protein antibody; EGFRBP GRB2 antibody; Epidermal growth factor receptor binding protein antibody; Epidermal growth factor receptor binding protein GRB2 antibody; GRB 2 antibody; GRB2 adapter protein antibody; Grb2 antibody; GRB2_HUMAN antibody; Grb3 3 antibody; Growth factor receptor bound protein 2 antibody; Growth factor receptor bound protein 3 antibody; Growth factor receptor-bound protein 2 antibody; HT027 antibody; MST084 antibody; MSTP084 antibody; NCKAP2 antibody; OTTHUMP00000166096 antibody; OTTHUMP00000166097 antibody; OTTHUMP00000166098 antibody; Protein ash antibody; SEM5 antibody; SH2/SH3 adapter GRB2 antibody
SEQUENCE SIMILARITIES
Belongs to the GRB2/sem-5/DRK family.
SUBCELLULAR LOCATION
Cytoplasm, nucleus.
FUNCTION
Grb2 is widely expressed and is essential for multiple cellular functions. Inhibition of Grb2 function impairs developmental processes in various organisms and blocks transformation and proliferation of various cell types, and so it is not surprising that a targeted gene disruption of Grb2 in mouse is lethal at an early embryonic stage. Grb2 is best known for its ability to link the epidermal growth factor receptor tyrosine kinase to the activation of Ras and its downstream kinases, ERK1,2. Grb2 is composed of an SH2 domain flanked on each side by an SH3 domain. Grb2 has two closely related proteins with similar domain organizations, Gads and Grap. Gads and Grap are expressed specifically in hematopoietic cells and function in the coordination of tyrosine kinase mediated signal transduction. The SH2 domain of Grb2 binds to phosphorylated tyrosine-containing peptides on receptors or scaffold proteins with a preference for pY-X-N-X, where X is generally a hydrophobic residue such as valine.