PRODUCT CODE: ER31203

GRB2 Antibody (ER31203)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of GRB2 on different cell lysates using anti-GRB2 antibody at 1/500 dilution.
  • Western blot analysis of GRB2 on different cell lysates using anti-GRB2 antibody at 1/500 dilution.
  • ICC staining GRB2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GRB2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-GRB2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SHG-44 cells with GRB2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of GRB2 on different cell lysates using anti-GRB2 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

GRB2 Antibody (ER31203)

Immunogen

Peptide

Host

Rabbit

Positive Control

F9, HepG2, SHG-44, human breast cancer tissue, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

25 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Growth factor receptor-bound protein 2

GENE NAME

GRB2

SYNONYMS

GRB2

SEQUENCE SIMILARITIES

Belongs to the GRB2/sem-5/DRK family.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Endosome. Golgi apparatus.

FUNCTION

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.; Isoform 2 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform 2 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.