PRODUCT CODE: M1701-8

GPX5 Monoclonal Antibody (M1701-8)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of GPX5 on human sperm tissue lysate using anti-GPX5 antibody at 1/5,000 dilution.
  • Western blot analysis of GPX5 on human sperm tissue lysate using anti-GPX5 antibody at 1/5,000 dilution.
  • Western blot analysis of GPX5 on mouse testis tissue lysate using anti-GPX5 antibody at 1/500 dilution.
  • ICC staining GPX5 (green) in 293T cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GPX5 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GPX5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GPX5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GPX5 antibody. Counter stained with hematoxylin.
Western blot analysis of GPX5 on human sperm tissue lysate using anti-GPX5 antibody at 1/5,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

GPX5 Monoclonal Antibody (M1701-8)

Immunogen

Peptide

Host

Mouse

Positive Control

Mouse testis tissue lysate, human sperm tissue lysate, 293T, Hela,  human liver tissue, mouse liver tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

5A1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

22 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

GPX5

SYNONYMS

Arep; arMEP24; EGLP; Epididymal androgen related protein; Epididymal secretory glutathione peroxidase; epididymis secretory sperm binding protein Li 75p; Epididymis specific glutathione peroxidase like protein; Epididymis-specific glutathione peroxidase-like protein; Glutathione peroxidase 5; Glutathione peroxidase 5 (epididymal androgen related protein); Glutathione peroxidase, epididymal; GPx-5; GPX5; GPX5_HUMAN; GSHPx-5; HEL-S-75p; Major androgen regulated protein; OTTHUMP00000017919; OTTHUMP00000214636

SEQUENCE SIMILARITIES

Belongs to the glutathione peroxidase family.

TISSUE SPECIFICITY

Epididymis.

SUBCELLULAR LOCATION

Secreted. 

FUNCTION

Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1-8). GPx-1, Gpx-2 and Gpx-3 exist as homotetramers. Gpx-4 has a high tendancy to form high molecular weight oligomers. GPx-1 plays an important role in the antioxidant defense of the vascular wall and neural cells in response to oxidative stress. GPx-2 is the major isoform in the lungs and its basal or inducible expression is dependent on Nrf2. GPx-3 is under regulation by hypoxic stress and the expression and deficiency of GPx-3 is associated with cardiovascular disease and stroke. GPx-5 is selenium-independent; it is bound to the acrosome of sperm, where it may protect sperm from premature acrosome reaction in the epididymis.