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PRODUCT CODE: ER1802-48

GOT1 Antibody (ER1802-48)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of GOT1 on K562 cell lysate using anti-GOT1 antibody at 1/500 dilution.
  • Western blot analysis of GOT1 on K562 cell lysate using anti-GOT1 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GOT1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-GOT1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-GOT1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with GOT1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of GOT1 on K562 cell lysate using anti-GOT1 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

GOT1 Antibody (ER1802-48)

Immunogen

Recombinant protein with human got1 aa 1-200.

Host

Rabbit

Positive Control

K562, human liver tissue, human colon tissue, human heart tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

46 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P17174

PROTEIN NAME

GOT1

SYNONYMS

CGI-141 antibody; GCT2 antibody; Germ cell tumor 2 antibody; Golgi transport 1 homolog B antibody; Golgi transport 1B antibody; GOLT1B antibody; GOT1 antibody; GOT1B_HUMAN antibody; hGOT1a antibody; Putative NF-kappa-B-activating protein 470 antibody; Vesicle transport protein GOT1B antibody; YMR292W antibody

SEQUENCE SIMILARITIES

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.