PRODUCT CODE: EM40708

Glutathione Peroxidase 1 Monoclonal Antibody (EM40708)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Positive control: Western blot analysis of GPX1 on different cell lysates using anti-GPX1 antibody at 1/1000 dilution.<br />
Positive control:<br />
Lane 1: THP-1<br />
Lane 2: HepG2<br />
Lane 3: 293T
  • Positive control: Western blot analysis of GPX1 on different cell lysates using anti-GPX1 antibody at 1/1000 dilution.<br />
Positive control:<br />
Lane 1: THP-1<br />
Lane 2: HepG2<br />
Lane 3: 293T
  • ICC staining GPX1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GPX1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GPX1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with GPX1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Positive control: Western blot analysis of GPX1 on different cell lysates using anti-GPX1 antibody at 1/1000 dilution.
Positive control:
Lane 1: THP-1
Lane 2: HepG2
Lane 3: 293T

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Glutathione Peroxidase 1 Monoclonal Antibody (EM40708)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Hela, HepG2, 293T, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

C5-F6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • ICC

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Glutathione peroxidase 1

GENE NAME

GPX1

SYNONYMS

GSHPx-1,GPx-1

SEQUENCE SIMILARITIES

Belongs to the glutathione peroxidase family.

TISSUE SPECIFICITY

Expressed in platelets (at protein level).

POST-TRANSLATIONAL MODIFICATION

During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Protects the hemoglobin in erythrocytes from oxidative breakdown. In platelets, plays a crucial role of glutathione peroxidase in the arachidonic acid metabolism.