PRODUCT CODE: EM40708

Glutathione Peroxidase 1 Monoclonal Antibody (EM40708)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Positive control: Western blot analysis of GPX1 on different cell lysates using anti-GPX1 antibody at 1/1000 dilution.<br />
 Positive control:<br />
 Lane 1: THP-1<br />
 Lane 2: HepG2<br />
 Lane 3: 293T
  • Positive control: Western blot analysis of GPX1 on different cell lysates using anti-GPX1 antibody at 1/1000 dilution.<br />
 Positive control:<br />
 Lane 1: THP-1<br />
 Lane 2: HepG2<br />
 Lane 3: 293T
  • ICC staining GPX1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GPX1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GPX1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with GPX1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Positive control: Western blot analysis of GPX1 on different cell lysates using anti-GPX1 antibody at 1/1000 dilution.
Positive control:
Lane 1: THP-1
Lane 2: HepG2
Lane 3: 293T

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Glutathione Peroxidase 1 Monoclonal Antibody (EM40708)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Hela, HepG2, 293T, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

C5-F6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • ICC

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Glutathione Peroxidase 1

SYNONYMS

AL033363 antibody; Cellular glutathione peroxidase antibody; Glutathione peroxidase 1 antibody; Glutathione peroxidase antibody; GPx 1 antibody; GPx-1 antibody; GPX1 antibody; GPX1_HUMAN antibody; GPXD antibody; GSHPx-1 antibody; GSHPX1 antibody; MGC14399 antibody; MGC88245 antibody

SEQUENCE SIMILARITIES

Belongs to the glutathione peroxidase family.

TISSUE SPECIFICITY

Expressed in platelets (at protein level).

POST-TRANSLATIONAL MODIFICATION

During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

GPX1 is ubiquitously expressed in many tissues, where it protects cells from oxidative stress. Within cells, it localizes to the cytoplasm and mitochondria. As a glutathione peroxidase, GPx1 functions in the detoxification of hydrogen peroxide, specifically by catalyzing the reduction of hydrogen peroxide to water. The glutathione peroxidase also catalyzes the reduction of other organic hydroperoxides, such as lipid peroxides, to the corresponding alcohols. GPx1 typically uses glutathione (GSH) as the reductant, but when glutathione synthetase (GSS) is, as in brain mitochondria, γ-glutamylcysteine can serve as the reductant instead. The protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell death and increases resistance to toxic challenges, especially oxidative stress. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.