PRODUCT CODE: EM1901-56

Glutathione peroxidase 1 Monoclonal Antibody (EM1901-56)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Glutathione peroxidase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: THP-1 cell lysate<br />
 Lane 2: 293 cell lysate
  • Western blot analysis of Glutathione peroxidase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: THP-1 cell lysate<br />
 Lane 2: 293 cell lysate
  • ICC staining of Glutathione peroxidase 1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-56, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Flow cytometric analysis of Glutathione peroxidase 1 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-56, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of Glutathione peroxidase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: THP-1 cell lysate
Lane 2: 293 cell lysate

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Glutathione peroxidase 1 Monoclonal Antibody (EM1901-56)

Immunogen

Recombinant protein within human gpx1 aa 30-203.

Host

Mouse

Positive Control

THP-1 cell lysate, 293 cell lysate, F9, THP-1.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

C5-A10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G affinity purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Glutathione peroxidase 1

SYNONYMS

AL033363 antibody; Cellular glutathione peroxidase antibody; Glutathione peroxidase 1 antibody; Glutathione peroxidase antibody; GPx 1 antibody; GPx-1 antibody; GPX1 antibody; GPX1_HUMAN antibody; GPXD antibody; GSHPx-1 antibody; GSHPX1 antibody; MGC14399 antibody; MGC88245 antibody

SEQUENCE SIMILARITIES

Belongs to the glutathione peroxidase family.

TISSUE SPECIFICITY

Expressed in platelets (at protein level).

POST-TRANSLATIONAL MODIFICATION

During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1-8). GPx-1, Gpx-2 and Gpx-3 exist as homotetramers. Gpx-4 has a high tendancy to form high molecular weight oligomers. GPx-1 plays an important role in the antioxidant defense of the vascular wall and neural cells in response to oxidative stress. GPx-2 is the major isoform in the lungs and its basal or inducible expression is dependent on Nrf2. GPx-3 is under regulation by hypoxic stress and the expression and deficiency of GPx-3 is associated with cardiovascular disease and stroke. GPx-5 is selenium-independent; it is bound to the acrosome of sperm, where it may protect sperm from premature acrosome reaction in the epididymis.